Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • M Opekarová
  • Thomas Caspari
    Lehstuhl für Zellbiologische und Pflanzenphysiologie, Universität Regensburg, Regensburg
  • Widmar Tanner
    Lehstuhl für Zellbiologische und Pflanzenphysiologie, Universität Regensburg, Regensburg

Amino acids are accumulated in Saccharomyces cerevisiae by strictly unidirectional influx systems. To see whether cellular compartmentation causes this unusual amino-acid-transport behaviour, arginine transport was studied in plasma-membrane vesicles. The arginine permease gene CAN1 was overexpressed in S. cerevisiae RH218a and in a permease-deficient mutant RS453 (can1). Reconstituted plasma-membrane vesicles from these transformants, energized by incorporated cytochrome-c oxidase, showed 3-4-fold increased rates of arginine uptake compared to vesicles from wild-type cells. The KT values were 32.5 microM in vesicles from wild-type and 28.6 microM in vesicles from transformed cells; the corresponding in vivo values were 17.5 microM and 11.4 microM, respectively. It could be demonstrated that unidirectional arginine transport and accumulation also exist in vesicles; thus, unidirectional influx is not related to cellular compartmentation.

Allweddeiriau

Iaith wreiddiolSaesneg
Tudalennau (o-i)683-8
Nifer y tudalennau6
CyfnodolynEuropean Journal of Biochemistry
Cyfrol211
Rhif y cyfnodolyn3
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Chwef 1993
Cyhoeddwyd yn allanolIe
Gweld graff cysylltiadau