Soils support a wide range of ecosystem services that underpin Earth system functioning. It is therefore essential that we have robust approaches to evaluate how anthropogenic perturbation affects soil quality and the delivery of these services. Metabolomics, the large-scale study of low molecular weight organic compounds in soil, offers one potential approach to characterise soils and evaluate the metabolic status of the soil biological community. The aims of the present study were to 1) characterise the soil metabolome across a contrasting range of soil types, 2) understand the relationships between common chemical and physical soil quality indicators and its metabolome, and 3) evaluate the discriminatory power of soil metabolomics and its potential use as a soil quality indicator.