TY - JOUR

T1 - A nanosecond pulsed electric field (nsPEF) can affect membrane permeabilization and cellular viability in a 3D spheroids tumor model

AU - Carr, Lynn

AU - Golzio, Muriel

AU - Orlacchio, Rosa

AU - Alberola, Geraldine

AU - Kolosnjaj-Tabi, Jelena

AU - Leveque, Philippe

AU - Arnaud-Cormos, Delia

AU - Rols, Marie-Pierre

N1 - Brought in from Pubs router with BU affiliation on paper, but after BU contract ended

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Three-dimensional (3D) cellular models represent more realistically the complexity of in vivo tumors compared to 2D cultures. While 3D models were largely used in classical electroporation, the effects of nanosecond pulsed electric field (nsPEF) have been poorly investigated. In this study, we evaluated the biological effects induced by nsPEF on spheroid tumor model derived from the HCT-116 human colorectal carcinoma cell line. By varying the number of pulses (from 1 to 500) and the polarity (unipolar and bipolar), the response of nsPEF exposure (10 ns duration, 50 kV/cm) was assessed either immediately after the application of the pulses or over a period lasting up to 6 days. Membrane permeabilization and cellular death occurred following the application of at least 100 pulses. The extent of the response increased with the number of pulses, with a significant decrease of viability, 24 h post-exposure, when 250 and 500 pulses were applied. The effects were highly reduced when an equivalent number of bipolar pulses were delivered. This reduction was eliminated when a 100 ns interphase interval was introduced into the bipolar pulses. Altogether, our results show that nsPEF effects, previously observed at the single cell level, also occur in more realistic 3D tumor spheroids models. [Abstract copyright: Copyright © 2021 Elsevier B.V. All rights reserved.]

AB - Three-dimensional (3D) cellular models represent more realistically the complexity of in vivo tumors compared to 2D cultures. While 3D models were largely used in classical electroporation, the effects of nanosecond pulsed electric field (nsPEF) have been poorly investigated. In this study, we evaluated the biological effects induced by nsPEF on spheroid tumor model derived from the HCT-116 human colorectal carcinoma cell line. By varying the number of pulses (from 1 to 500) and the polarity (unipolar and bipolar), the response of nsPEF exposure (10 ns duration, 50 kV/cm) was assessed either immediately after the application of the pulses or over a period lasting up to 6 days. Membrane permeabilization and cellular death occurred following the application of at least 100 pulses. The extent of the response increased with the number of pulses, with a significant decrease of viability, 24 h post-exposure, when 250 and 500 pulses were applied. The effects were highly reduced when an equivalent number of bipolar pulses were delivered. This reduction was eliminated when a 100 ns interphase interval was introduced into the bipolar pulses. Altogether, our results show that nsPEF effects, previously observed at the single cell level, also occur in more realistic 3D tumor spheroids models. [Abstract copyright: Copyright © 2021 Elsevier B.V. All rights reserved.]

KW - 3D cell culture

KW - Bipolar cancellation

KW - Electropulsation

KW - Nanosecond pulsed electric field (nsPEF)

KW - Spheroids

U2 - 10.1016/j.bioelechem.2021.107839

DO - 10.1016/j.bioelechem.2021.107839

M3 - Article

C2 - 34020398

VL - 141

JO - Bioelectrochemistry (Amsterdam, Netherlands)

JF - Bioelectrochemistry (Amsterdam, Netherlands)

SN - 1878-562X

M1 - 107839

ER -