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A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway

Research output: Contribution to journal › Article › peer-review

Standard Standard

A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. / Marchetti, Maria A; Kumar, Sanjay; Hartsuiker, Edgar et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 11, 28.05.2002, p. 7472-7.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Marchetti, MA, Kumar, S, Hartsuiker, E, Maftahi, M, Carr, AM, Freyer, GA, Burhans, WC & Huberman, JA 2002, 'A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 11, pp. 7472-7. https://doi.org/10.1073/pnas.112702399

APA

Marchetti, M. A., Kumar, S., Hartsuiker, E., Maftahi, M., Carr, A. M., Freyer, G. A., Burhans, W. C., & Huberman, J. A. (2002). A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proceedings of the National Academy of Sciences of the United States of America, 99(11), 7472-7. https://doi.org/10.1073/pnas.112702399

CBE

Marchetti MA, Kumar S, Hartsuiker E, Maftahi M, Carr AM, Freyer GA, Burhans WC, Huberman JA. 2002. A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proceedings of the National Academy of Sciences of the United States of America. 99(11):7472-7. https://doi.org/10.1073/pnas.112702399

MLA

Marchetti, Maria A et al. "A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway". Proceedings of the National Academy of Sciences of the United States of America. 2002, 99(11). 7472-7. https://doi.org/10.1073/pnas.112702399

VancouverVancouver

Marchetti MA, Kumar S, Hartsuiker E, Maftahi M, Carr AM, Freyer GA et al. A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proceedings of the National Academy of Sciences of the United States of America. 2002 May 28;99(11):7472-7. doi: 10.1073/pnas.112702399

Author

Marchetti, Maria A ; Kumar, Sanjay ; Hartsuiker, Edgar et al. / A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 11. pp. 7472-7.

RIS

TY - JOUR

T1 - A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway

AU - Marchetti, Maria A

AU - Kumar, Sanjay

AU - Hartsuiker, Edgar

AU - Maftahi, Mohamed

AU - Carr, Antony M

AU - Freyer, Greg A

AU - Burhans, William C

AU - Huberman, Joel A

PY - 2002/5/28

Y1 - 2002/5/28

N2 - The eukaryotic intra-S-phase checkpoint, which slows DNA synthesis in response to DNA damage, is poorly understood. Is DNA damage recognized directly, or indirectly through its effects on replication forks? Is the slowing of S phase in part because of competition between DNA synthesis and recombination/repair processes? The results of our genetic analyses of the intra-S-phase checkpoint in the fission yeast, Schizosaccharomyces pombe, suggest that the slowing of S phase depends weakly on the helicases Rqh1 and Srs2 but not on other recombination/repair pathways. The slowing of S phase depends strongly on the six checkpoint-Rad proteins, on Cds1, and on Rad4/Cut5 (similar to budding yeast Dpb11, which interacts with DNA polymerase epsilon) but not on Rhp9 (similar to budding yeast Rad9, necessary for direct damage recognition). These results suggest that, in fission yeast, the signal activating the intra-S-phase checkpoint is generated only when replication forks encounter DNA damage.

AB - The eukaryotic intra-S-phase checkpoint, which slows DNA synthesis in response to DNA damage, is poorly understood. Is DNA damage recognized directly, or indirectly through its effects on replication forks? Is the slowing of S phase in part because of competition between DNA synthesis and recombination/repair processes? The results of our genetic analyses of the intra-S-phase checkpoint in the fission yeast, Schizosaccharomyces pombe, suggest that the slowing of S phase depends weakly on the helicases Rqh1 and Srs2 but not on other recombination/repair pathways. The slowing of S phase depends strongly on the six checkpoint-Rad proteins, on Cds1, and on Rad4/Cut5 (similar to budding yeast Dpb11, which interacts with DNA polymerase epsilon) but not on Rhp9 (similar to budding yeast Rad9, necessary for direct damage recognition). These results suggest that, in fission yeast, the signal activating the intra-S-phase checkpoint is generated only when replication forks encounter DNA damage.

KW - Cell Cycle Proteins

KW - DNA Damage

KW - DNA Repair

KW - DNA Repair Enzymes

KW - DNA Replication

KW - DNA-Binding Proteins

KW - Endonucleases

KW - Humans

KW - Nuclear Proteins

KW - Proliferating Cell Nuclear Antigen

KW - Recombination, Genetic

KW - S Phase

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

U2 - 10.1073/pnas.112702399

DO - 10.1073/pnas.112702399

M3 - Article

C2 - 12032307

VL - 99

SP - 7472

EP - 7477

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -