TY - JOUR

T1 - Acidithiobacillus sulfuriphilus sp. nov.

T2 - an extremely acidophilic sulfur-oxidizing chemolithotroph isolated from a neutral pH environment

AU - Falagán, Carmen

AU - Moya-Beltrán, Ana

AU - Castro, Matías

AU - Quatrini, Raquel

AU - Johnson, D Barrie

N1 - This work was funded by the Natural Environment Research Council, UK (Grant reference NE/L014076/1)

PY - 2019/9/1

Y1 - 2019/9/1

N2 - The genus Acidithiobacillus currently includes seven species with validly published names, which fall into two major groups, those that can oxidize ferrous iron and those that do not. All seven species can use zero-valent sulfur and reduced sulfur oxy-anions as electron donors, are obligately chemolithotrophic and acidophilic bacteria with pH growth optima below 3.0. The 16S rRNA gene of a novel strain (CJ-2T) isolated from circum-neutral pH mine drainage showed 95-97 % relatedness to members of the genus Acidithiobacillus. Digital DNA-DNA hybridization (dDDH) values between strains and whole-genome pairwise comparisons between the CJ-2T strain and the reference genomes available for members of the genus Acidithiobacillus confirmed that CJ-2Trepresents a novel species of this genus. CJ-2T is a strict aerobe, oxidizes zero-valent sulfur and reduced inorganic sulfur compounds but does not use ferrous iron or hydrogen as electron donors. The isolate is mesophilic (optimum growth temperature 25-28 °C) and extremely acidophilic (optimum growth pH 3.0), though its pH optimum and maximum were significantly higher than those of non-iron-oxidising acidithiobacilli with validly published names. The major fatty acids of CJ-2T were C18 : 1ω7c, C:16 : 1ω7c/iso-C15 : 0 2-OH, C16 : 0 and C19 : 0 cyclo ω8c and the major respiratory quinone present was Q8. The name Acidithiobacillussulfuriphilus sp. nov. is proposed, the type strain is CJ-2T (=DSM 105150T=KCTC 4683T).

AB - The genus Acidithiobacillus currently includes seven species with validly published names, which fall into two major groups, those that can oxidize ferrous iron and those that do not. All seven species can use zero-valent sulfur and reduced sulfur oxy-anions as electron donors, are obligately chemolithotrophic and acidophilic bacteria with pH growth optima below 3.0. The 16S rRNA gene of a novel strain (CJ-2T) isolated from circum-neutral pH mine drainage showed 95-97 % relatedness to members of the genus Acidithiobacillus. Digital DNA-DNA hybridization (dDDH) values between strains and whole-genome pairwise comparisons between the CJ-2T strain and the reference genomes available for members of the genus Acidithiobacillus confirmed that CJ-2Trepresents a novel species of this genus. CJ-2T is a strict aerobe, oxidizes zero-valent sulfur and reduced inorganic sulfur compounds but does not use ferrous iron or hydrogen as electron donors. The isolate is mesophilic (optimum growth temperature 25-28 °C) and extremely acidophilic (optimum growth pH 3.0), though its pH optimum and maximum were significantly higher than those of non-iron-oxidising acidithiobacilli with validly published names. The major fatty acids of CJ-2T were C18 : 1ω7c, C:16 : 1ω7c/iso-C15 : 0 2-OH, C16 : 0 and C19 : 0 cyclo ω8c and the major respiratory quinone present was Q8. The name Acidithiobacillussulfuriphilus sp. nov. is proposed, the type strain is CJ-2T (=DSM 105150T=KCTC 4683T).

KW - Acidithiobacillus/classification

KW - Bacterial Typing Techniques

KW - Base Composition

KW - DNA, Bacterial/genetics

KW - Fatty Acids/chemistry

KW - Hydrogen-Ion Concentration

KW - Iron

KW - Mining

KW - Nucleic Acid Hybridization

KW - Oxidation-Reduction

KW - Phylogeny

KW - RNA, Ribosomal, 16S/genetics

KW - Sequence Analysis, DNA

KW - Sulfur/metabolism

KW - Wales

KW - Water Microbiology

U2 - 10.1099/ijsem.0.003576

DO - 10.1099/ijsem.0.003576

M3 - Article

C2 - 31274405

VL - 69

SP - 2907

EP - 2913

JO - International Journal of Systematic Evolutionary Microbiology

JF - International Journal of Systematic Evolutionary Microbiology

SN - 1466-5034

IS - 9

ER -