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Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae

Research output: Contribution to journal › Article › peer-review

Standard Standard

Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae. / Raj, Kaushik; Partow, Siavash; Correia, Kevin et al.
In: Metabolic engineering communications, Vol. 6, 01.06.2018, p. 28-32.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Raj, K, Partow, S, Correia, K, Khusnutdinova, AN, Yakunin, AF & Mahadevan, R 2018, 'Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae', Metabolic engineering communications, vol. 6, pp. 28-32. https://doi.org/10.1016/j.meteno.2018.02.001

APA

Raj, K., Partow, S., Correia, K., Khusnutdinova, A. N., Yakunin, A. F., & Mahadevan, R. (2018). Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae. Metabolic engineering communications, 6, 28-32. https://doi.org/10.1016/j.meteno.2018.02.001

CBE

Raj K, Partow S, Correia K, Khusnutdinova AN, Yakunin AF, Mahadevan R. 2018. Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae. Metabolic engineering communications. 6:28-32. https://doi.org/10.1016/j.meteno.2018.02.001

MLA

Raj, Kaushik et al. "Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae". Metabolic engineering communications. 2018, 6. 28-32. https://doi.org/10.1016/j.meteno.2018.02.001

VancouverVancouver

Raj K, Partow S, Correia K, Khusnutdinova AN, Yakunin AF, Mahadevan R. Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae. Metabolic engineering communications. 2018 Jun 1;6:28-32. Epub 2018 Feb 3. doi: 10.1016/j.meteno.2018.02.001

Author

Raj, Kaushik ; Partow, Siavash ; Correia, Kevin et al. / Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae. In: Metabolic engineering communications. 2018 ; Vol. 6. pp. 28-32.

RIS

TY - JOUR

T1 - Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae

AU - Raj, Kaushik

AU - Partow, Siavash

AU - Correia, Kevin

AU - Khusnutdinova, Anna N

AU - Yakunin, Alexander F

AU - Mahadevan, Radhakrishnan

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks.

AB - Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks.

U2 - 10.1016/j.meteno.2018.02.001

DO - 10.1016/j.meteno.2018.02.001

M3 - Article

C2 - 29487800

VL - 6

SP - 28

EP - 32

JO - Metabolic engineering communications

JF - Metabolic engineering communications

SN - 2214-0301

ER -