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Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods

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Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods. / Whiteley, N.M.; Magney, J.L.; McCleary, S.J. et al.
In: Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, Vol. 157, No. 2, 01.10.2010, p. 116-122.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Whiteley, NM, Magney, JL, McCleary, SJ, Nia, SK, El Haj, AJ & Rock, J 2010, 'Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods', Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, vol. 157, no. 2, pp. 116-122. https://doi.org/10.1016/j.cbpa.2010.05.014

APA

Whiteley, N. M., Magney, J. L., McCleary, S. J., Nia, S. K., El Haj, A. J., & Rock, J. (2010). Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 157(2), 116-122. https://doi.org/10.1016/j.cbpa.2010.05.014

CBE

Whiteley NM, Magney JL, McCleary SJ, Nia SK, El Haj AJ, Rock J. 2010. Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 157(2):116-122. https://doi.org/10.1016/j.cbpa.2010.05.014

MLA

Whiteley, N.M. et al. "Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods". Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 2010, 157(2). 116-122. https://doi.org/10.1016/j.cbpa.2010.05.014

VancouverVancouver

Whiteley NM, Magney JL, McCleary SJ, Nia SK, El Haj AJ, Rock J. Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 2010 Oct 1;157(2):116-122. doi: 10.1016/j.cbpa.2010.05.014

Author

Whiteley, N.M. ; Magney, J.L. ; McCleary, S.J. et al. / Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods. In: Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 2010 ; Vol. 157, No. 2. pp. 116-122.

RIS

TY - JOUR

T1 - Characterisation of myosin heavy chain gene variants in the fast and slow muscle fibres of gammarid amphipods

AU - Whiteley, N.M.

AU - Magney, J.L.

AU - McCleary, S.J.

AU - Nia, S.K.

AU - El Haj, A.J.

AU - Rock, J.

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Recent molecular work has revealed a large diversity of myosin heavy chain (MyHC) gene variants in the abdominal musculature of gammarid amphipods. An unusual truncated MyHC transcript from the loop 1 region (Variant A3) was consistently observed in multiple species and populations. The current study aimed to determine whether this MyHC variant is specific to a particular muscle fibre type, as a change in net charge to the loop 1 region of Variant A3 could be functionally significant. The localisation of different fibre types within the abdominal musculature of several gammarid species revealed that the deep flexor and extensor muscles are fast-twitch muscle fibres. The dorsal superficial muscles were identified as slow fibres and the muscles extrinsic to the pleopods were identified as intermediate fibres. Amplification of loop 1 region mRNA from isolated superficial extensor and deep flexor muscles, and subsequent liquid chromatography and sequence analysis revealed that Variant A3 was the primary MyHC variant in slow muscles, and the conserved A1 sequence was the primary variant in fast muscles. The specific role of Variant A3 in the slow muscles remains to be investigated.

AB - Recent molecular work has revealed a large diversity of myosin heavy chain (MyHC) gene variants in the abdominal musculature of gammarid amphipods. An unusual truncated MyHC transcript from the loop 1 region (Variant A3) was consistently observed in multiple species and populations. The current study aimed to determine whether this MyHC variant is specific to a particular muscle fibre type, as a change in net charge to the loop 1 region of Variant A3 could be functionally significant. The localisation of different fibre types within the abdominal musculature of several gammarid species revealed that the deep flexor and extensor muscles are fast-twitch muscle fibres. The dorsal superficial muscles were identified as slow fibres and the muscles extrinsic to the pleopods were identified as intermediate fibres. Amplification of loop 1 region mRNA from isolated superficial extensor and deep flexor muscles, and subsequent liquid chromatography and sequence analysis revealed that Variant A3 was the primary MyHC variant in slow muscles, and the conserved A1 sequence was the primary variant in fast muscles. The specific role of Variant A3 in the slow muscles remains to be investigated.

U2 - 10.1016/j.cbpa.2010.05.014

DO - 10.1016/j.cbpa.2010.05.014

M3 - Article

VL - 157

SP - 116

EP - 122

JO - Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology

SN - 1095-6433

IS - 2

ER -