TY - JOUR

T1 - Complex target SELEX-based identification of DNA aptamers against Bungarus caeruleus venom for the detection of envenomation using a paper-based device

AU - Anand, Anjali

AU - Chatterjee, Bandhan

AU - Dhiman, Abhijeet

AU - Goel, Renu

AU - Khan, Eshan

AU - Malhotra, Anita

AU - Santra, Vishal

AU - Salvi, Nitin

AU - Bhatnagar, Ira

AU - Kumar, Amit

AU - Asthana, Amit

AU - Sharma, Tarun Kumar

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is β-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.

AB - Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is β-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.

KW - Aptamers

KW - SELEX

KW - Krait

KW - Snake venom

KW - Bungarotoxin

KW - Mass-spectrometry

KW - Paper device

U2 - 10.1016/j.bios.2021.113523

DO - 10.1016/j.bios.2021.113523

M3 - Article

VL - 193

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

M1 - 113523

ER -