TY - JOUR

T1 - Confocal quality imaging of afferent neurons from semi-thin sections of Drosophila ganglia

AU - Tyrer, N. Mark

AU - Maddison, John

AU - Shepherd, David

AU - Williams, Darren W.

PY - 2000/12/22

Y1 - 2000/12/22

N2 - The aim of this study was to develop protocols for computer imaging of the thoraco-abdominal ganglion of Drosophila from serial semi-thin sections, in which specific neurons were stained and related to neuropilar structures. The central projections of a subset of transgenically labelled sensory neurons were revealed by immunohistochemistry, while Nomarski optics were used to show motor neuron targets in the neuropil. Digital photomicrographs of each section were aligned and the resultant image stacks rendered into three-dimensional (3D) images that can be rotated in real time. The result is a detailed, in-depth visualization of labelled neurons at a resolution comparable with that in confocal reconstructions, which also allows investigation of their relationships with other components of the neuropil.

AB - The aim of this study was to develop protocols for computer imaging of the thoraco-abdominal ganglion of Drosophila from serial semi-thin sections, in which specific neurons were stained and related to neuropilar structures. The central projections of a subset of transgenically labelled sensory neurons were revealed by immunohistochemistry, while Nomarski optics were used to show motor neuron targets in the neuropil. Digital photomicrographs of each section were aligned and the resultant image stacks rendered into three-dimensional (3D) images that can be rotated in real time. The result is a detailed, in-depth visualization of labelled neurons at a resolution comparable with that in confocal reconstructions, which also allows investigation of their relationships with other components of the neuropil.

KW - Animals

KW - Animals, Genetically Modified/anatomy & histology

KW - Central Nervous System/cytology

KW - Coloring Agents

KW - Drosophila melanogaster/cytology

KW - Ganglia, Invertebrate/cytology

KW - Genes, Reporter/physiology

KW - Green Fluorescent Proteins

KW - Image Processing, Computer-Assisted/instrumentation

KW - Indicators and Reagents/metabolism

KW - Luminescent Proteins/metabolism

KW - Microscopy, Confocal/instrumentation

KW - Microtomy/methods

KW - Neurons, Afferent/cytology

M3 - Article

C2 - 11108989

VL - 296

SP - 93

EP - 96

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -