A compact test structure and setup for ns-electroporation of biological cells is described. A CPW with defected ground assures impedance match with low dispersion or parasitic. The defected ground forms a microchamber for quick cell manipulation. The measured results along with EM analysis show that a 1-ns 0.1-V transmembrane potential can be induced. Although this is below the threshold for electroporation, it can be increased by using pulses with 1-ns and 10-, or 10-ns and 3-times higher amplitude.