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CRISPR-induced DNA reorganization for multiplexed nucleic acid detection

Research output: Contribution to journal › Article › peer-review

Standard Standard

CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. / Karlikow, Margot; Amalfitano, Evan; Yang, Xiaolong et al.
In: Nature Communications, Vol. 14, No. 1, 1505, 17.03.2023.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Karlikow, M, Amalfitano, E, Yang, X, Doucet, J, Chapman, A, Sadat-Moussavi, P, Homme, P, Sutyrina, P, Chang, W, Lemak, S, Yakunin, A, Dolezal, AG, Kelley, S, Foster, L, Harpur, B & Pardee, K 2023, 'CRISPR-induced DNA reorganization for multiplexed nucleic acid detection', Nature Communications, vol. 14, no. 1, 1505. https://doi.org/10.1038/s41467-023-36874-6

APA

Karlikow, M., Amalfitano, E., Yang, X., Doucet, J., Chapman, A., Sadat-Moussavi, P., Homme, P., Sutyrina, P., Chang, W., Lemak, S., Yakunin, A., Dolezal, A. G., Kelley, S., Foster, L., Harpur, B., & Pardee, K. (2023). CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. Nature Communications, 14(1), Article 1505. https://doi.org/10.1038/s41467-023-36874-6

CBE

Karlikow M, Amalfitano E, Yang X, Doucet J, Chapman A, Sadat-Moussavi P, Homme P, Sutyrina P, Chang W, Lemak S, et al. 2023. CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. Nature Communications. 14(1):Article 1505. https://doi.org/10.1038/s41467-023-36874-6

MLA

Karlikow, Margot et al. "CRISPR-induced DNA reorganization for multiplexed nucleic acid detection". Nature Communications. 2023. 14(1). https://doi.org/10.1038/s41467-023-36874-6

VancouverVancouver

Karlikow M, Amalfitano E, Yang X, Doucet J, Chapman A, Sadat-Moussavi P et al. CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. Nature Communications. 2023 Mar 17;14(1):1505. Epub 2023 Mar 17. doi: 10.1038/s41467-023-36874-6

Author

Karlikow, Margot ; Amalfitano, Evan ; Yang, Xiaolong et al. / CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. In: Nature Communications. 2023 ; Vol. 14, No. 1.

RIS

TY - JOUR

T1 - CRISPR-induced DNA reorganization for multiplexed nucleic acid detection

AU - Karlikow, Margot

AU - Amalfitano, Evan

AU - Yang, Xiaolong

AU - Doucet, Jennifer

AU - Chapman, Abigail

AU - Sadat-Moussavi, Peivand

AU - Homme, Paige

AU - Sutyrina, Polina

AU - Chang, Winston

AU - Lemak, Sofia

AU - Yakunin, Alexander

AU - Dolezal, Adam G.

AU - Kelley, Shana

AU - Foster, Leonard

AU - Harpur, Brock

AU - Pardee, Keith

N1 - © 2023. The Author(s).

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Nucleic acid sensing powered by the sequence recognition of CRIPSR technologies has enabled major advancement toward rapid, accurate and deployable diagnostics. While exciting, there are still many challenges facing their practical implementation, such as the widespread need for a PAM sequence in the targeted nucleic acid, labile RNA inputs, and limited multiplexing. Here we report FACT (Functionalized Amplification CRISPR Tracing), a CRISPR-based nucleic acid barcoding technology compatible with Cas12a and Cas13a, enabling diagnostic outputs based on cis- and trans-cleavage from any sequence. Furthermore, we link the activation of CRISPR-Cas12a to the expression of proteins through a Reprogrammable PAIRing system (RePAIR). We then combine FACT and RePAIR to create FACTOR (FACT on RePAIR), a CRISPR-based diagnostic, that we use to detect infectious disease in an agricultural use case: honey bee viral infection. With high specificity and accuracy, we demonstrate the potential of FACTOR to be applied to the sensing of any nucleic acid of interest.

AB - Nucleic acid sensing powered by the sequence recognition of CRIPSR technologies has enabled major advancement toward rapid, accurate and deployable diagnostics. While exciting, there are still many challenges facing their practical implementation, such as the widespread need for a PAM sequence in the targeted nucleic acid, labile RNA inputs, and limited multiplexing. Here we report FACT (Functionalized Amplification CRISPR Tracing), a CRISPR-based nucleic acid barcoding technology compatible with Cas12a and Cas13a, enabling diagnostic outputs based on cis- and trans-cleavage from any sequence. Furthermore, we link the activation of CRISPR-Cas12a to the expression of proteins through a Reprogrammable PAIRing system (RePAIR). We then combine FACT and RePAIR to create FACTOR (FACT on RePAIR), a CRISPR-based diagnostic, that we use to detect infectious disease in an agricultural use case: honey bee viral infection. With high specificity and accuracy, we demonstrate the potential of FACTOR to be applied to the sensing of any nucleic acid of interest.

U2 - 10.1038/s41467-023-36874-6

DO - 10.1038/s41467-023-36874-6

M3 - Article

C2 - 36932065

VL - 14

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1505

ER -