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Detection of covalent DNA-bound Spo11 and topoisomerase complexes

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Detection of covalent DNA-bound Spo11 and topoisomerase complexes. / Hartsuiker, E.; Tsubouchi, H. (Editor).
DNA recombination: Methods and Protocols. Springer Science and Business Media LLC, 2011. p. 65-77.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

HarvardHarvard

Hartsuiker, E & Tsubouchi, H (ed.) 2011, Detection of covalent DNA-bound Spo11 and topoisomerase complexes. in DNA recombination: Methods and Protocols. Springer Science and Business Media LLC, pp. 65-77. https://doi.org/10.1007/978-1-61779-129-1_5

APA

Hartsuiker, E., & Tsubouchi, H. (Ed.) (2011). Detection of covalent DNA-bound Spo11 and topoisomerase complexes. In DNA recombination: Methods and Protocols (pp. 65-77). Springer Science and Business Media LLC. https://doi.org/10.1007/978-1-61779-129-1_5

CBE

Hartsuiker E, Tsubouchi H, ed. 2011. Detection of covalent DNA-bound Spo11 and topoisomerase complexes. In DNA recombination: Methods and Protocols. Springer Science and Business Media LLC. pp. 65-77. https://doi.org/10.1007/978-1-61779-129-1_5

MLA

Hartsuiker, E. and H. Tsubouchi, ed. "Detection of covalent DNA-bound Spo11 and topoisomerase complexes". DNA recombination: Methods and Protocols. Springer Science and Business Media LLC. 2011, 65-77. https://doi.org/10.1007/978-1-61779-129-1_5

VancouverVancouver

Hartsuiker E, Tsubouchi H, (ed.). Detection of covalent DNA-bound Spo11 and topoisomerase complexes. In DNA recombination: Methods and Protocols. Springer Science and Business Media LLC. 2011. p. 65-77 doi: 10.1007/978-1-61779-129-1_5

Author

Hartsuiker, E. ; Tsubouchi, H. (Editor). / Detection of covalent DNA-bound Spo11 and topoisomerase complexes. DNA recombination: Methods and Protocols. Springer Science and Business Media LLC, 2011. pp. 65-77

RIS

TY - CHAP

T1 - Detection of covalent DNA-bound Spo11 and topoisomerase complexes

AU - Hartsuiker, E.

A2 - Tsubouchi, H.

PY - 2011/4/28

Y1 - 2011/4/28

N2 - Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA

AB - Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA

U2 - 10.1007/978-1-61779-129-1_5

DO - 10.1007/978-1-61779-129-1_5

M3 - Chapter

SN - 9781617791291

SP - 65

EP - 77

BT - DNA recombination: Methods and Protocols

PB - Springer Science and Business Media LLC

ER -