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Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery

Research output: Contribution to journal › Article › peer-review

Standard Standard

Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery. / Cude, M.P.; Gwenin, C.D.
In: ECS Transactions, Vol. 33, No. 38, 01.01.2011, p. 79-89.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Cude, MP & Gwenin, CD 2011, 'Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery', ECS Transactions, vol. 33, no. 38, pp. 79-89. https://doi.org/10.1149/1.3583517

APA

Cude, M. P., & Gwenin, C. D. (2011). Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery. ECS Transactions, 33(38), 79-89. https://doi.org/10.1149/1.3583517

CBE

Cude MP, Gwenin CD. 2011. Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery. ECS Transactions. 33(38):79-89. https://doi.org/10.1149/1.3583517

MLA

Cude, M.P. and C.D. Gwenin. "Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery". ECS Transactions. 2011, 33(38). 79-89. https://doi.org/10.1149/1.3583517

VancouverVancouver

Cude MP, Gwenin CD. Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery. ECS Transactions. 2011 Jan 1;33(38):79-89. doi: 10.1149/1.3583517

Author

Cude, M.P. ; Gwenin, C.D. / Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery. In: ECS Transactions. 2011 ; Vol. 33, No. 38. pp. 79-89.

RIS

TY - JOUR

T1 - Development of Gold Coated Superparamagnetic Iron Oxide Nanoparticles for Nitroreductase Delivery

AU - Cude, M.P.

AU - Gwenin, C.D.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys12 can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.

AB - Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys12 can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.

U2 - 10.1149/1.3583517

DO - 10.1149/1.3583517

M3 - Article

VL - 33

SP - 79

EP - 89

JO - ECS Transactions

JF - ECS Transactions

SN - 1938-6737

IS - 38

ER -