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Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw

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Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw. / Ward, Rhys A.; Charlton, Adam; Welham, Kevin J. et al.
In: Electrochemistry Communications, Vol. 124, 106942, 03.2021.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Ward, RA, Charlton, A, Welham, KJ, Baker, P, Zein, SH, Tomkinson, J, Richards, DI, Kelly, SM, Lawrence, NS & Wadhawan, JD 2021, 'Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw', Electrochemistry Communications, vol. 124, 106942. https://doi.org/10.1016/j.elecom.2021.106942

APA

Ward, R. A., Charlton, A., Welham, K. J., Baker, P., Zein, S. H., Tomkinson, J., Richards, D. I., Kelly, S. M., Lawrence, N. S., & Wadhawan, J. D. (2021). Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw. Electrochemistry Communications, 124, Article 106942. https://doi.org/10.1016/j.elecom.2021.106942

CBE

Ward RA, Charlton A, Welham KJ, Baker P, Zein SH, Tomkinson J, Richards DI, Kelly SM, Lawrence NS, Wadhawan JD. 2021. Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw. Electrochemistry Communications. 124:Article 106942. https://doi.org/10.1016/j.elecom.2021.106942

MLA

Ward, Rhys A. et al. "Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw". Electrochemistry Communications. 2021. 124. https://doi.org/10.1016/j.elecom.2021.106942

VancouverVancouver

Ward RA, Charlton A, Welham KJ, Baker P, Zein SH, Tomkinson J et al. Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw. Electrochemistry Communications. 2021 Mar;124:106942. Epub 2021 Feb 4. doi: 10.1016/j.elecom.2021.106942

Author

Ward, Rhys A. ; Charlton, Adam ; Welham, Kevin J. et al. / Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw. In: Electrochemistry Communications. 2021 ; Vol. 124.

RIS

TY - JOUR

T1 - Electrochemical quantification of D-glucose during the production of bioethanol from thermo-mechanically pre-treated wheat straw

AU - Ward, Rhys A.

AU - Charlton, Adam

AU - Welham, Kevin J.

AU - Baker, Paul

AU - Zein, Sharif H.

AU - Tomkinson, Jeremy

AU - Richards, David I.

AU - Kelly, Stephen M.

AU - Lawrence, Nathan S.

AU - Wadhawan, Jay D.

N1 - © 2021 The Author(s).

PY - 2021/3

Y1 - 2021/3

N2 - Mechanical pre-treatment (disc refining) of wheat straw, at both atmospheric and elevated pressure, is shown to be an efficient process to access fermentable monosaccharides, with the potential to integrate within the infrastructure of existing first-generation bioethanol plants.The mild, enzymatic degradation of this sustainable lignocellulosic biomass affords ca. 0.10-0.13 g/g (dry weight) of D-glucose quantifiable voltammetrically in real time, over a two hundred-fold range in experimental laboratory scales (25 mL to 5.0 L), with pressure discrefining of the wheat straw enabling almost twice the amount of D-glucose to be generated during the hydrolysis stage than experiments using atmospheric refining (0.06 – 0.09 g/g dry weight). Fermentation of the resulting hydrolysate affords 0.08 – 0.10 g/g (dry weight) of ethanol over similar scales, with ethanol productivity at ca. 37 mg/(L h). These results demonstrate that minimal cellulose decomposition occurs during pressure refining of wheat straw, in contrast to hemicellulose, and suggest that the development of green,mechanochemical processes for the scalable and cost-effective manufacture of secondgeneration bioethanol requires improved cellulose decomposition.

AB - Mechanical pre-treatment (disc refining) of wheat straw, at both atmospheric and elevated pressure, is shown to be an efficient process to access fermentable monosaccharides, with the potential to integrate within the infrastructure of existing first-generation bioethanol plants.The mild, enzymatic degradation of this sustainable lignocellulosic biomass affords ca. 0.10-0.13 g/g (dry weight) of D-glucose quantifiable voltammetrically in real time, over a two hundred-fold range in experimental laboratory scales (25 mL to 5.0 L), with pressure discrefining of the wheat straw enabling almost twice the amount of D-glucose to be generated during the hydrolysis stage than experiments using atmospheric refining (0.06 – 0.09 g/g dry weight). Fermentation of the resulting hydrolysate affords 0.08 – 0.10 g/g (dry weight) of ethanol over similar scales, with ethanol productivity at ca. 37 mg/(L h). These results demonstrate that minimal cellulose decomposition occurs during pressure refining of wheat straw, in contrast to hemicellulose, and suggest that the development of green,mechanochemical processes for the scalable and cost-effective manufacture of secondgeneration bioethanol requires improved cellulose decomposition.

KW - Bioethanol

KW - Lignocellulosic biomass

KW - pre-treatment

KW - What straw

KW - Thermomechanical refining

KW - Glucose oxidase

KW - Mechanochemistry

U2 - 10.1016/j.elecom.2021.106942

DO - 10.1016/j.elecom.2021.106942

M3 - Article

C2 - 33767578

VL - 124

JO - Electrochemistry Communications

JF - Electrochemistry Communications

M1 - 106942

ER -