Standard Standard

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression. / Diep, Patrick; Leo Shen, Heping; Wiesner, Julian A et al.
In: Engineering in life sciences, Vol. 23, No. 7, 2200133, 07.2023.

Research output: Contribution to journalArticlepeer-review

HarvardHarvard

Diep, P, Leo Shen, H, Wiesner, JA, Mykytczuk, N, Papangelakis, V, Yakunin, AF & Mahadevan, R 2023, 'Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression', Engineering in life sciences, vol. 23, no. 7, 2200133. https://doi.org/10.1002/elsc.202200133

APA

Diep, P., Leo Shen, H., Wiesner, J. A., Mykytczuk, N., Papangelakis, V., Yakunin, A. F., & Mahadevan, R. (2023). Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression. Engineering in life sciences, 23(7), Article 2200133. https://doi.org/10.1002/elsc.202200133

CBE

Diep P, Leo Shen H, Wiesner JA, Mykytczuk N, Papangelakis V, Yakunin AF, Mahadevan R. 2023. Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression. Engineering in life sciences. 23(7):Article 2200133. https://doi.org/10.1002/elsc.202200133

MLA

VancouverVancouver

Diep P, Leo Shen H, Wiesner JA, Mykytczuk N, Papangelakis V, Yakunin AF et al. Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression. Engineering in life sciences. 2023 Jul;23(7):2200133. Epub 2023 May 24. doi: 10.1002/elsc.202200133

Author

Diep, Patrick ; Leo Shen, Heping ; Wiesner, Julian A et al. / Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression. In: Engineering in life sciences. 2023 ; Vol. 23, No. 7.

RIS

TY - JOUR

T1 - Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

AU - Diep, Patrick

AU - Leo Shen, Heping

AU - Wiesner, Julian A

AU - Mykytczuk, Nadia

AU - Papangelakis, Vladimiros

AU - Yakunin, Alexander F

AU - Mahadevan, Radhakrishnan

N1 - © 2023 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH.

PY - 2023/7

Y1 - 2023/7

N2 - Mine wastewater often contains dissolved metals at concentrations too low to be economically extracted by existing technologies, yet too high for environmental discharge. The most common treatment is chemical precipitation of the dissolved metals using limestone and subsequent disposal of the sludge in tailing impoundments. While it is a cost-effective solution to meet regulatory standards, it represents a lost opportunity. In this study, we engineered Escherichia coli to overexpress its native NikABCDE transporter and a heterologous metallothionein to capture nickel at concentrations in local effluent streams. We found the engineered strain had a 7-fold improvement in the bioaccumulation performance for nickel compared to controls, but also observed a drastic decrease in cell viability due to metabolic burden or inducer (IPTG) toxicity. Growth kinetic analysis revealed the IPTG concentrations used based on past studies lead to growth inhibition, thus delineating future avenues for optimization of the engineered strain and its growth conditions to perform in more complex environments.

AB - Mine wastewater often contains dissolved metals at concentrations too low to be economically extracted by existing technologies, yet too high for environmental discharge. The most common treatment is chemical precipitation of the dissolved metals using limestone and subsequent disposal of the sludge in tailing impoundments. While it is a cost-effective solution to meet regulatory standards, it represents a lost opportunity. In this study, we engineered Escherichia coli to overexpress its native NikABCDE transporter and a heterologous metallothionein to capture nickel at concentrations in local effluent streams. We found the engineered strain had a 7-fold improvement in the bioaccumulation performance for nickel compared to controls, but also observed a drastic decrease in cell viability due to metabolic burden or inducer (IPTG) toxicity. Growth kinetic analysis revealed the IPTG concentrations used based on past studies lead to growth inhibition, thus delineating future avenues for optimization of the engineered strain and its growth conditions to perform in more complex environments.

U2 - 10.1002/elsc.202200133

DO - 10.1002/elsc.202200133

M3 - Article

C2 - 37408871

VL - 23

JO - Engineering in life sciences

JF - Engineering in life sciences

SN - 1618-0240

IS - 7

M1 - 2200133

ER -