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DOI

  • Sofia Bonilla
    Department of Chemical Engineering and Applied Chemistry at the University of Toronto
  • Zahra Choolaei
    Department of Chemical Engineering and Applied Chemistry at the University of Toronto
  • Torsten Meyer
    Department of Chemical Engineering and Applied Chemistry at the University of Toronto
  • Elizabeth A Edwards
    Department of Chemical Engineering and Applied Chemistry at the University of Toronto
  • Alexander F Yakunin
    Department of Chemical Engineering and Applied Chemistry at the University of Toronto
  • D Grant Allen
    Department of Chemical Engineering and Applied Chemistry at the University of Toronto

Anaerobic digestion of biosludge has not yet been implemented in pulp mills due to low biogas yields. Enzymatic pretreatment of biosludge has shown improvements in biogas yields but results are varied. A key limitation of previous studies is that they fail to consider the COD contribution from the enzyme solutions. The aim of this study was to systematically investigate the potential for enzymatic pretreatment on the anaerobic digestibility of pulp mill biosludge. Out of the six enzymes tested, four enhanced the anaerobic digestibility of biosludge. At the end of the BMP, a maximum improvement of 26% in biogas yield was observed with protease from B. licheniformis. There was no correlation between enzymatic activities on standard substrates and/or on biosludge and the effect of enzymes on biogas yields. Enzymes have potential for improving biosludge anaerobic digestibility but more research on optimal conditions and potential synergies with other pretreatment is needed.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalBiotechnology reports (Amsterdam, Netherlands)
Volume17
Early online date5 Jan 2018
DOIs
Publication statusPublished - 1 Mar 2018
Externally publishedYes
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