TY - JOUR

T1 - First mitogenome phylogeny of the sun bear Helarctos malayanus reveals a deep split between Indochinese and Sundaic lineages

AU - Kunde, Miriam N.

AU - Barlow, Axel

AU - Klittich, Achim M.

AU - Yakupova, Aliya

AU - Fickel, Jorns

AU - Forster, Daniel W.

PY - 2023/7/9

Y1 - 2023/7/9

N2 - The sun bear Helarctos malayanus is one of the most endangered ursids, and to date classification of sun bear populations has been based almost exclusively on geographic distribution and morphology. The very few molecular studies focussing on this spe -cies were limited in geographic scope. Using archival and non- invasively collected sample material, we have added a substantial number of complete or near- complete mitochondrial genome sequences from sun bears of several range countries of the species' distribution. We here report 32 new mitogenome sequences represent-ing sun bears from Cambodia, Thailand, Peninsular Malaysia, Sumatra, and Borneo. Reconstruction of phylogenetic relationships revealed two matrilines that diverged ~295 thousand years ago: one restricted to portions of mainland Indochina (China, Cambodia, Thailand; “Mainland clade”), and one comprising bears from Borneo, Sumatra, Peninsular Malaysia but also Thailand (“Sunda clade”). Generally recent coa -lescence times in the mitochondrial phylogeny suggest that recent or historical demo -graphic processes have resulted in a loss of mtDNA variation. Additionally, analysis of our data in conjunction with shorter mtDNA sequences revealed that the Bornean sun bear, classified as a distinct subspecies (H. m. euryspilus), does not harbor a distinc -tive matriline. Further molecular studies of H. malayanus are needed, which should ideally include data from nuclear loci.

AB - The sun bear Helarctos malayanus is one of the most endangered ursids, and to date classification of sun bear populations has been based almost exclusively on geographic distribution and morphology. The very few molecular studies focussing on this spe -cies were limited in geographic scope. Using archival and non- invasively collected sample material, we have added a substantial number of complete or near- complete mitochondrial genome sequences from sun bears of several range countries of the species' distribution. We here report 32 new mitogenome sequences represent-ing sun bears from Cambodia, Thailand, Peninsular Malaysia, Sumatra, and Borneo. Reconstruction of phylogenetic relationships revealed two matrilines that diverged ~295 thousand years ago: one restricted to portions of mainland Indochina (China, Cambodia, Thailand; “Mainland clade”), and one comprising bears from Borneo, Sumatra, Peninsular Malaysia but also Thailand (“Sunda clade”). Generally recent coa -lescence times in the mitochondrial phylogeny suggest that recent or historical demo -graphic processes have resulted in a loss of mtDNA variation. Additionally, analysis of our data in conjunction with shorter mtDNA sequences revealed that the Bornean sun bear, classified as a distinct subspecies (H. m. euryspilus), does not harbor a distinc -tive matriline. Further molecular studies of H. malayanus are needed, which should ideally include data from nuclear loci.

U2 - 10.1002/ece3.9969

DO - 10.1002/ece3.9969

M3 - Article

VL - 13

JO - Ecology and Evolution

JF - Ecology and Evolution

SN - 2045-7758

IS - 4

ER -