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Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect.

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Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect. / Toubiana , William ; Dumas, Zoe; Tran Van, Patrick et al.
In: Science Advances, Vol. 11, No. 1, eads6459, 01.01.2025.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Toubiana , W, Dumas, Z, Tran Van, P, Parker, D, Mérel, V, Schubert , V, Aury , J-M, Bournonville , L, Cruaud, C, Houben , A, Istace, B, Labadie, K, Noel, B & Schwander, T 2025, 'Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect.', Science Advances, vol. 11, no. 1, eads6459. https://doi.org/10.1126/sciadv.ads6459

APA

Toubiana , W., Dumas, Z., Tran Van, P., Parker, D., Mérel, V., Schubert , V., Aury , J.-M., Bournonville , L., Cruaud, C., Houben , A., Istace, B., Labadie, K., Noel, B., & Schwander, T. (2025). Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect. Science Advances, 11(1), Article eads6459. https://doi.org/10.1126/sciadv.ads6459

CBE

Toubiana W, Dumas Z, Tran Van P, Parker D, Mérel V, Schubert V, Aury J-M, Bournonville L, Cruaud C, Houben A, et al. 2025. Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect. Science Advances. 11(1):Article eads6459. https://doi.org/10.1126/sciadv.ads6459

MLA

Toubiana , William et al. "Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect.". Science Advances. 2025. 11(1). https://doi.org/10.1126/sciadv.ads6459

VancouverVancouver

Toubiana W, Dumas Z, Tran Van P, Parker D, Mérel V, Schubert V et al. Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect. Science Advances. 2025 Jan 1;11(1):eads6459. doi: 10.1126/sciadv.ads6459

Author

Toubiana , William ; Dumas, Zoe ; Tran Van, Patrick et al. / Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect. In: Science Advances. 2025 ; Vol. 11, No. 1.

RIS

TY - JOUR

T1 - Functional monocentricity with holocentric characteristics and chromosome-specific centromeres in a stick insect.

AU - Toubiana , William

AU - Dumas, Zoe

AU - Tran Van, Patrick

AU - Parker, Darren

AU - Mérel, Vincent

AU - Schubert , Veit

AU - Aury , Jean-Marc

AU - Bournonville , Lorène

AU - Cruaud, Corinne

AU - Houben , Andreas

AU - Istace, Benjamin

AU - Labadie, Karine

AU - Noel, Benjamin

AU - Schwander, Tanja

PY - 2025/1/1

Y1 - 2025/1/1

N2 - Centromeres are essential for chromosome segregation in eukaryotes, yet their specification is unexpectedly diverse among species and can involve major transitions such as those from localized to chromosome-wide centromeres between monocentric and holocentric species. How this diversity evolves remains elusive. We discovered within-cell variation in the recruitment of the major centromere protein CenH3, reminiscent of variation typically observed among species. While CenH3-containing nucleosomes are distributed in a monocentric fashion on autosomes and bind tandem repeat sequences specific to individual or groups of chromosomes, they show a longitudinal distribution and broad intergenic binding on the X chromosome, which partially recapitulates phenotypes known from holocentric species. Despite this variable CenH3 distribution among chromosomes, all chromosomes are functionally monocentric, marking the first instance of a monocentric species with chromosome-wide CenH3 deposition. Together, our findings illustrate a potential transitional state between mono- and holocentricity or toward CenH3-independent centromere determination and help to understand the rapid centromere sequence divergence between species.

AB - Centromeres are essential for chromosome segregation in eukaryotes, yet their specification is unexpectedly diverse among species and can involve major transitions such as those from localized to chromosome-wide centromeres between monocentric and holocentric species. How this diversity evolves remains elusive. We discovered within-cell variation in the recruitment of the major centromere protein CenH3, reminiscent of variation typically observed among species. While CenH3-containing nucleosomes are distributed in a monocentric fashion on autosomes and bind tandem repeat sequences specific to individual or groups of chromosomes, they show a longitudinal distribution and broad intergenic binding on the X chromosome, which partially recapitulates phenotypes known from holocentric species. Despite this variable CenH3 distribution among chromosomes, all chromosomes are functionally monocentric, marking the first instance of a monocentric species with chromosome-wide CenH3 deposition. Together, our findings illustrate a potential transitional state between mono- and holocentricity or toward CenH3-independent centromere determination and help to understand the rapid centromere sequence divergence between species.

U2 - 10.1126/sciadv.ads6459

DO - 10.1126/sciadv.ads6459

M3 - Article

VL - 11

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 1

M1 - eads6459

ER -