Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator.
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n laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.
| Original language | English |
|---|---|
| Journal | Cold Spring Harbor Protocols |
| Volume | 2018 |
| Issue number | 2 |
| Early online date | 21 Jul 2017 |
| DOIs | |
| Publication status | Published - Feb 2018 |