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Label-free graphene biosensor targeting cancer molecules based on non-covalent modification

Research output: Contribution to journal › Article › peer-review

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Label-free graphene biosensor targeting cancer molecules based on non-covalent modification. / Zhou, L. ; Mao, H.; Wu, C. et al.
In: Biosensors and Bioelectronics, Vol. 87, 15.01.2018, p. 701-707.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Zhou, L, Mao, H, Wu, C, Tang, L, Wu, Z, Sun, H, Zhang, H, Zhou, H, Jia, C, Jin, Q, Chen, X & Zhao, J 2018, 'Label-free graphene biosensor targeting cancer molecules based on non-covalent modification', Biosensors and Bioelectronics, vol. 87, pp. 701-707. https://doi.org/10.1016/j.bios.2016.09.025

APA

Zhou, L., Mao, H., Wu, C., Tang, L., Wu, Z., Sun, H., Zhang, H., Zhou, H., Jia, C., Jin, Q., Chen, X., & Zhao, J. (2018). Label-free graphene biosensor targeting cancer molecules based on non-covalent modification. Biosensors and Bioelectronics, 87, 701-707. https://doi.org/10.1016/j.bios.2016.09.025

CBE

Zhou L, Mao H, Wu C, Tang L, Wu Z, Sun H, Zhang H, Zhou H, Jia C, Jin Q, et al. 2018. Label-free graphene biosensor targeting cancer molecules based on non-covalent modification. Biosensors and Bioelectronics. 87:701-707. https://doi.org/10.1016/j.bios.2016.09.025

MLA

Zhou, L. et al. "Label-free graphene biosensor targeting cancer molecules based on non-covalent modification". Biosensors and Bioelectronics. 2018, 87. 701-707. https://doi.org/10.1016/j.bios.2016.09.025

VancouverVancouver

Zhou L, Mao H, Wu C, Tang L, Wu Z, Sun H et al. Label-free graphene biosensor targeting cancer molecules based on non-covalent modification. Biosensors and Bioelectronics. 2018 Jan 15;87:701-707. Epub 2016 Sept 8. doi: 10.1016/j.bios.2016.09.025

Author

Zhou, L. ; Mao, H. ; Wu, C. et al. / Label-free graphene biosensor targeting cancer molecules based on non-covalent modification. In: Biosensors and Bioelectronics. 2018 ; Vol. 87. pp. 701-707.

RIS

TY - JOUR

T1 - Label-free graphene biosensor targeting cancer molecules based on non-covalent modification

AU - Zhou, L.

AU - Mao, H.

AU - Wu, C.

AU - Tang, L.

AU - Wu, Z.

AU - Sun, H.

AU - Zhang, H.

AU - Zhou, H.

AU - Jia, C.

AU - Jin, Q.

AU - Chen, Xianfeng

AU - Zhao, J.

PY - 2018/1/15

Y1 - 2018/1/15

N2 - A label-free immunosensor based on antibody-modified graphene field effect transistor (GFET) was presented. Antibodies targeting carcinoembryonic antigen (Anti-CEA) were immobilized to the graphene surface via non-covalent modification. The bifunctional molecule, 1-pyrenebutanoic acid succinimidyl ester, which is composed of a pyrene and a reactive succinimide ester group, interacts with graphene non-covalently via π-stacking. The succinimide ester group reacts with the amine group to initiate antibody surface immobilization, which was confirmed by X-ray Photoelectron Spectroscopy, Atomic Force Microscopy and Electrochemical Impedance Spectroscopy. The resulting anti-CEA modified GFET sufficiently monitored the reaction between CEA protein and anti-CEA in real-time with high specificity, which revealed selective electrical detection of CEA with a limit of detection (LOD) of less than 100 pg/ml. The dissociation constant between CEA protein and anti-CEA was estimated to be 6.35×10−11 M, indicating the high affinity and sensitivity of anti-CEA-GFET. Taken together, the graphene biosensors provide an effective tool for clinical application and point-of-care medical diagnostics.

AB - A label-free immunosensor based on antibody-modified graphene field effect transistor (GFET) was presented. Antibodies targeting carcinoembryonic antigen (Anti-CEA) were immobilized to the graphene surface via non-covalent modification. The bifunctional molecule, 1-pyrenebutanoic acid succinimidyl ester, which is composed of a pyrene and a reactive succinimide ester group, interacts with graphene non-covalently via π-stacking. The succinimide ester group reacts with the amine group to initiate antibody surface immobilization, which was confirmed by X-ray Photoelectron Spectroscopy, Atomic Force Microscopy and Electrochemical Impedance Spectroscopy. The resulting anti-CEA modified GFET sufficiently monitored the reaction between CEA protein and anti-CEA in real-time with high specificity, which revealed selective electrical detection of CEA with a limit of detection (LOD) of less than 100 pg/ml. The dissociation constant between CEA protein and anti-CEA was estimated to be 6.35×10−11 M, indicating the high affinity and sensitivity of anti-CEA-GFET. Taken together, the graphene biosensors provide an effective tool for clinical application and point-of-care medical diagnostics.

U2 - 10.1016/j.bios.2016.09.025

DO - 10.1016/j.bios.2016.09.025

M3 - Article

VL - 87

SP - 701

EP - 707

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -