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Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin. / Choolaei, Zahra; Flick, Robert; Khusnutdinova, Anna et al.
In: Journal of Biotechnology, Vol. 325, 10.01.2021, p. 128-137.

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Choolaei Z, Flick R, Khusnutdinova A, Edwards EA, Yakunin AF. Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin. Journal of Biotechnology. 2021 Jan 10;325:128-137. Epub 2020 Nov 10. doi: 10.1016/j.jbiotec.2020.11.007

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Choolaei, Zahra ; Flick, Robert ; Khusnutdinova, Anna et al. / Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin. In: Journal of Biotechnology. 2021 ; Vol. 325. pp. 128-137.

RIS

TY - JOUR

T1 - Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin

AU - Choolaei, Zahra

AU - Flick, Robert

AU - Khusnutdinova, Anna

AU - Edwards, Elizabeth A.

AU - Yakunin, Alexander F

N1 - Copyright © 2020 Elsevier B.V. All rights reserved.

PY - 2021/1/10

Y1 - 2021/1/10

N2 - Efficient biotransformation of lignin requires the activity of different oxidative enzymes. In this work, 19 bacterial multi-copper oxidases were screened for oxidase activity against 19 soluble substrates and revealed the highest activity in the laccase CotABsu (BSU0630) from Bacillus subtilis. Structure-based site-directed mutagenesis of CotABsu identified four conserved residues (His419, Cys492, His497, and Met502) as critical for activity against 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Greatly reduced oxidase activity was found in the CotABsu mutant proteins E213A, N214A, C229A, N264A, E298A, T415A, R416A, Q468A, and T480A. We also designed a lignin-agarose plate screen for detecting oxidase activity of purified proteins against polymeric lignin, which confirmed the results obtained with ABTS and identified three mutant variants with increased activity toward kraft lignin (E213A, T415A, and T260A). X-ray photoelectron spectroscopy analysis of low sulfonate kraft lignin after incubation with CotABsu revealed a reduction in the content of CC/CC bonds and increase in CO/CO bonds. Product analyses using mass spectrometry, liquid chromatography, and bright-field microscopy revealed an increased polymerization state of reaction products suggesting that formation of radical intermediates was followed by radical coupling. Our results provide further insights into the mechanisms of lignin oxidation by laccases.

AB - Efficient biotransformation of lignin requires the activity of different oxidative enzymes. In this work, 19 bacterial multi-copper oxidases were screened for oxidase activity against 19 soluble substrates and revealed the highest activity in the laccase CotABsu (BSU0630) from Bacillus subtilis. Structure-based site-directed mutagenesis of CotABsu identified four conserved residues (His419, Cys492, His497, and Met502) as critical for activity against 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Greatly reduced oxidase activity was found in the CotABsu mutant proteins E213A, N214A, C229A, N264A, E298A, T415A, R416A, Q468A, and T480A. We also designed a lignin-agarose plate screen for detecting oxidase activity of purified proteins against polymeric lignin, which confirmed the results obtained with ABTS and identified three mutant variants with increased activity toward kraft lignin (E213A, T415A, and T260A). X-ray photoelectron spectroscopy analysis of low sulfonate kraft lignin after incubation with CotABsu revealed a reduction in the content of CC/CC bonds and increase in CO/CO bonds. Product analyses using mass spectrometry, liquid chromatography, and bright-field microscopy revealed an increased polymerization state of reaction products suggesting that formation of radical intermediates was followed by radical coupling. Our results provide further insights into the mechanisms of lignin oxidation by laccases.

KW - Bacillus subtilis/metabolism

KW - Laccase/genetics

KW - Lignin/metabolism

KW - Mutagenesis, Site-Directed

KW - Oxidation-Reduction

U2 - 10.1016/j.jbiotec.2020.11.007

DO - 10.1016/j.jbiotec.2020.11.007

M3 - Article

C2 - 33186661

VL - 325

SP - 128

EP - 137

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -