TY - JOUR

T1 - Long-term farmyard manure application affects soil organic phosphorus cycling: A combined metagenomic and P-33/C-14 labelling study

AU - Ma, Qingxu

AU - Wen, Yuan

AU - Ma, Jinzhao

AU - Macdonald, Andy

AU - Hill, Paul W.

AU - Chadwick, David R.

AU - Wu, Lianghuan

AU - Jones, Davey L.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Maintaining an adequate phosphorus (P) supply for plants and microorganisms is central to agricultural production; however, the long-term effects of organic manure and inorganic fertilizer application on soil P cycling remain unclear. Organic P cycling in a sandy loam soil receiving medium and high rates of farmyard manure (FYM) with and without mineral fertilisers was studied in a long-term field experiment with 14C/33P isotope labelling and metagenomic shotgun sequencing. FYM application alone negatively affected soil total P and organic P (Po) accumulation by enhancing crop offtake, enhancing Po mineralisation and stimulating P loss from the topsoil by reducing its P sorption potential. The P mineralisation/immobilisation rates detected by the 33P pool dilution method were significantly correlated with the abundance of microbial P cycling genes. Soil available C and N concentrations were related to gross P mineralisation/immobilisation rates and the abundance of P uptake/scavenging genes. Microbial genes related to P uptake and metabolism were more abundant than P scavenging genes, while P scavenging genes may work efficiently as both of them can sustain similar P mineralisation and immobilisation rates. The addition of FYM also promoted phosphatase activity reflecting the increased supply of Po in these soils. Our study demonstrates that long-term FYM application alters soil Po stocks and cycling, and that microbial functional gene abundance was coupled with P cycling rates.

AB - Maintaining an adequate phosphorus (P) supply for plants and microorganisms is central to agricultural production; however, the long-term effects of organic manure and inorganic fertilizer application on soil P cycling remain unclear. Organic P cycling in a sandy loam soil receiving medium and high rates of farmyard manure (FYM) with and without mineral fertilisers was studied in a long-term field experiment with 14C/33P isotope labelling and metagenomic shotgun sequencing. FYM application alone negatively affected soil total P and organic P (Po) accumulation by enhancing crop offtake, enhancing Po mineralisation and stimulating P loss from the topsoil by reducing its P sorption potential. The P mineralisation/immobilisation rates detected by the 33P pool dilution method were significantly correlated with the abundance of microbial P cycling genes. Soil available C and N concentrations were related to gross P mineralisation/immobilisation rates and the abundance of P uptake/scavenging genes. Microbial genes related to P uptake and metabolism were more abundant than P scavenging genes, while P scavenging genes may work efficiently as both of them can sustain similar P mineralisation and immobilisation rates. The addition of FYM also promoted phosphatase activity reflecting the increased supply of Po in these soils. Our study demonstrates that long-term FYM application alters soil Po stocks and cycling, and that microbial functional gene abundance was coupled with P cycling rates.

KW - Long-term fertilisation

KW - Phosphorus cycling

KW - Soil layers

KW - Substrate sorption

KW - Waste management and recycling

U2 - 10.1016/j.soilbio.2020.107959

DO - 10.1016/j.soilbio.2020.107959

M3 - Article

VL - 149

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

M1 - 107959

ER -