Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra
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In: Current Biology, 20.04.2015, p. R326-R327.
Research output: Contribution to journal › Article › peer-review
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T1 - Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra
AU - O'Neill, J.S.
AU - Lee, K.D.
AU - Zhang, L.
AU - Feeney, K.
AU - Webster, S.G.
AU - Blades, M.J.
AU - Kyriacou, C.P.
AU - Hastings, M.H.
AU - Wilcockson, D.C.
PY - 2015/4/20
Y1 - 2015/4/20
N2 - In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra. Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra, in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.
AB - In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra. Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra, in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.
U2 - 10.1016/j.cub.2015.02.052
DO - 10.1016/j.cub.2015.02.052
M3 - Article
SP - R326-R327
JO - Current Biology
JF - Current Biology
SN - 0960-9822
ER -