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Metagenomic approaches to the discovery of cellulases

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Metagenomic approaches to the discovery of cellulases. / Rooks, David J.; McDonald, James E; McCarthy, Alan J.
In: Methods in Enzymology, Vol. 510, 2012, p. 375-94.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Rooks, DJ, McDonald, JE & McCarthy, AJ 2012, 'Metagenomic approaches to the discovery of cellulases', Methods in Enzymology, vol. 510, pp. 375-94. https://doi.org/10.1016/B978-0-12-415931-0.00020-3

APA

Rooks, D. J., McDonald, J. E., & McCarthy, A. J. (2012). Metagenomic approaches to the discovery of cellulases. Methods in Enzymology, 510, 375-94. https://doi.org/10.1016/B978-0-12-415931-0.00020-3

CBE

Rooks DJ, McDonald JE, McCarthy AJ. 2012. Metagenomic approaches to the discovery of cellulases. Methods in Enzymology. 510:375-94. https://doi.org/10.1016/B978-0-12-415931-0.00020-3

MLA

Rooks, David J., James E McDonald and Alan J. McCarthy. "Metagenomic approaches to the discovery of cellulases". Methods in Enzymology. 2012, 510. 375-94. https://doi.org/10.1016/B978-0-12-415931-0.00020-3

VancouverVancouver

Rooks DJ, McDonald JE, McCarthy AJ. Metagenomic approaches to the discovery of cellulases. Methods in Enzymology. 2012;510:375-94. doi: 10.1016/B978-0-12-415931-0.00020-3

Author

Rooks, David J. ; McDonald, James E ; McCarthy, Alan J. / Metagenomic approaches to the discovery of cellulases. In: Methods in Enzymology. 2012 ; Vol. 510. pp. 375-94.

RIS

TY - JOUR

T1 - Metagenomic approaches to the discovery of cellulases

AU - Rooks, David J.

AU - McDonald, James E

AU - McCarthy, Alan J.

N1 - Copyright © 2012 Elsevier Inc. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Most of the microorganisms responsible for nutrient cycling in the environment have yet to be cultivated, and this could include those species responsible for the degradation of cellulose. Known cellulases are well defined at the protein sequence level, but gene variants are difficult to amplify from environmental DNA. The identification of novel cellulase genes independent of DNA amplification is made possible by adopting a direct metagenome sequencing approach to provide genes that can be cloned, expressed, and characterized prior to potential exploitation, all in the absence of any information on the species from which they originated. In this chapter, emerging strategies and methods that will enable the identification of novel cellulase genes and provide an unbiased perspective on gene expression in situ are presented.

AB - Most of the microorganisms responsible for nutrient cycling in the environment have yet to be cultivated, and this could include those species responsible for the degradation of cellulose. Known cellulases are well defined at the protein sequence level, but gene variants are difficult to amplify from environmental DNA. The identification of novel cellulase genes independent of DNA amplification is made possible by adopting a direct metagenome sequencing approach to provide genes that can be cloned, expressed, and characterized prior to potential exploitation, all in the absence of any information on the species from which they originated. In this chapter, emerging strategies and methods that will enable the identification of novel cellulase genes and provide an unbiased perspective on gene expression in situ are presented.

KW - Bacteria

KW - Cellulases

KW - DNA

KW - Fungi

KW - Gene Expression Profiling

KW - Gene Library

KW - Metagenomics

KW - RNA

KW - Transduction, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/B978-0-12-415931-0.00020-3

DO - 10.1016/B978-0-12-415931-0.00020-3

M3 - Article

C2 - 22608737

VL - 510

SP - 375

EP - 394

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

ER -