TY - JOUR

T1 - Methods for the isolation of cellulose-degrading microorganisms

AU - McDonald, James E

AU - Rooks, David J.

AU - McCarthy, Alan J.

N1 - Copyright © 2012 Elsevier Inc. All rights reserved.

PY - 2012

Y1 - 2012

N2 - The biodegradation of lignocellulose, the most abundant organic material in the biosphere, is a feature of many aerobic, facultatively anaerobic and obligately anaerobic bacteria and fungi. Despite widely recognized difficulties in the isolation and cultivation of individual microbial species from complex microbial populations and environments, significant progress has been made in recovering cellulolytic taxa from a range of ecological niches including the human, herbivore, and termite gut, and terrestrial, aquatic, and managed environments. Knowledge of cellulose-degrading microbial taxa is of significant importance with respect to nutrition, biodegradation, biotechnology, and the carbon-cycle, providing insights into the metabolism, physiology, and functional enzyme systems of the cellulolytic bacteria and fungi that are responsible for the largest flow of carbon in the biosphere. In this chapter, several strategies employed for the isolation and cultivation of cellulolytic microorganisms from oxic and anoxic environments are described.

AB - The biodegradation of lignocellulose, the most abundant organic material in the biosphere, is a feature of many aerobic, facultatively anaerobic and obligately anaerobic bacteria and fungi. Despite widely recognized difficulties in the isolation and cultivation of individual microbial species from complex microbial populations and environments, significant progress has been made in recovering cellulolytic taxa from a range of ecological niches including the human, herbivore, and termite gut, and terrestrial, aquatic, and managed environments. Knowledge of cellulose-degrading microbial taxa is of significant importance with respect to nutrition, biodegradation, biotechnology, and the carbon-cycle, providing insights into the metabolism, physiology, and functional enzyme systems of the cellulolytic bacteria and fungi that are responsible for the largest flow of carbon in the biosphere. In this chapter, several strategies employed for the isolation and cultivation of cellulolytic microorganisms from oxic and anoxic environments are described.

KW - Animals

KW - Bacteria

KW - Bacteria, Aerobic

KW - Bacteria, Anaerobic

KW - Bioreactors

KW - Cell Culture Techniques

KW - Cellulase

KW - Cellulose

KW - Enzyme Assays

KW - Feces

KW - Fungi

KW - Humans

KW - Isoptera

KW - Microbiological Techniques

KW - Rumen

KW - Sewage

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/B978-0-12-415931-0.00019-7

DO - 10.1016/B978-0-12-415931-0.00019-7

M3 - Article

C2 - 22608736

VL - 510

SP - 349

EP - 374

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

ER -