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Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa

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Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. / Bernardo, Pauline; Muhire, Brejnev; Francois, Sarah et al.
In: Virology, Vol. 493, 01.06.2016, p. 142-153.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Bernardo, P, Muhire, B, Francois, S, Deshoux, M, Hartnady, P, Farkas, K, Kraberger, S, Filloux, D, Fernandez, E, Galzi, S, Ferdinand, R, Granier, M, Marais, A, Blasco, PM, Candresse, T, Escriu, F, Varsani, A, Harkins, GW, Martin, DP & Roumagnac, P 2016, 'Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa', Virology, vol. 493, pp. 142-153. https://doi.org/10.1016/j.virol.2016.03.016

APA

Bernardo, P., Muhire, B., Francois, S., Deshoux, M., Hartnady, P., Farkas, K., Kraberger, S., Filloux, D., Fernandez, E., Galzi, S., Ferdinand, R., Granier, M., Marais, A., Blasco, P. M., Candresse, T., Escriu, F., Varsani, A., Harkins, G. W., Martin, D. P., & Roumagnac, P. (2016). Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virology, 493, 142-153. https://doi.org/10.1016/j.virol.2016.03.016

CBE

Bernardo P, Muhire B, Francois S, Deshoux M, Hartnady P, Farkas K, Kraberger S, Filloux D, Fernandez E, Galzi S, et al. 2016. Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virology. 493:142-153. https://doi.org/10.1016/j.virol.2016.03.016

MLA

Bernardo, Pauline et al. "Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa". Virology. 2016, 493. 142-153. https://doi.org/10.1016/j.virol.2016.03.016

VancouverVancouver

Bernardo P, Muhire B, Francois S, Deshoux M, Hartnady P, Farkas K et al. Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virology. 2016 Jun 1;493:142-153. Epub 2016 Mar 31. doi: 10.1016/j.virol.2016.03.016

Author

Bernardo, Pauline ; Muhire, Brejnev ; Francois, Sarah et al. / Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. In: Virology. 2016 ; Vol. 493. pp. 142-153.

RIS

TY - JOUR

T1 - Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa

AU - Bernardo, Pauline

AU - Muhire, Brejnev

AU - Francois, Sarah

AU - Deshoux, Maëlle

AU - Hartnady, Penelope

AU - Farkas, Kata

AU - Kraberger, Simona

AU - Filloux, Denis

AU - Fernandez, Emmanuel

AU - Galzi, Serge

AU - Ferdinand, Romain

AU - Granier, Martine

AU - Marais, Armelle

AU - Blasco, Pablo Monge

AU - Candresse, Thierry

AU - Escriu, Fernando

AU - Varsani, Arvind

AU - Harkins, Gordon W

AU - Martin, Darren P

AU - Roumagnac, Philippe

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.

AB - Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.

U2 - 10.1016/j.virol.2016.03.016

DO - 10.1016/j.virol.2016.03.016

M3 - Article

VL - 493

SP - 142

EP - 153

JO - Virology

JF - Virology

SN - 0042-6822

ER -