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Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi. / Keyes, Sam; van Veelen, Arjen; Fletcher, Dan McKay et al.
In: New Phytologist, Vol. 234, No. 2, 01.04.2022, p. 688-703.

Research output: Contribution to journalArticlepeer-review

HarvardHarvard

Keyes, S, van Veelen, A, Fletcher, DM, Scotson, C, Koebernick, N, Petroselli, C, Williams, K, Ruiz, S, Cooper, L, Mayon, R, Duncan, S, Dumont, M, Jakobsen, I, Oldroyd, G, Tkacz, A, Poole, P, Mosselmans, F, Borca, C, Huthwelker, T, Jones, DL & Roose, T 2022, 'Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi', New Phytologist, vol. 234, no. 2, pp. 688-703. https://doi.org/10.1111/nph.17980

APA

Keyes, S., van Veelen, A., Fletcher, D. M., Scotson, C., Koebernick, N., Petroselli, C., Williams, K., Ruiz, S., Cooper, L., Mayon, R., Duncan, S., Dumont, M., Jakobsen, I., Oldroyd, G., Tkacz, A., Poole, P., Mosselmans, F., Borca, C., Huthwelker, T., ... Roose, T. (2022). Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi. New Phytologist, 234(2), 688-703. https://doi.org/10.1111/nph.17980

CBE

Keyes S, van Veelen A, Fletcher DM, Scotson C, Koebernick N, Petroselli C, Williams K, Ruiz S, Cooper L, Mayon R, et al. 2022. Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi. New Phytologist. 234(2):688-703. https://doi.org/10.1111/nph.17980

MLA

VancouverVancouver

Keyes S, van Veelen A, Fletcher DM, Scotson C, Koebernick N, Petroselli C et al. Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi. New Phytologist. 2022 Apr 1;234(2):688-703. Epub 2022 Jan 19. doi: 10.1111/nph.17980

Author

Keyes, Sam ; van Veelen, Arjen ; Fletcher, Dan McKay et al. / Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi. In: New Phytologist. 2022 ; Vol. 234, No. 2. pp. 688-703.

RIS

TY - JOUR

T1 - Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi

AU - Keyes, Sam

AU - van Veelen, Arjen

AU - Fletcher, Dan McKay

AU - Scotson, Callum

AU - Koebernick, Nico

AU - Petroselli, Chiara

AU - Williams, Katherine

AU - Ruiz, Siul

AU - Cooper, Laura

AU - Mayon, Robbie

AU - Duncan, Simon

AU - Dumont, Marc

AU - Jakobsen, Iver

AU - Oldroyd, Giles

AU - Tkacz, Andrzej

AU - Poole, Philip

AU - Mosselmans, Fred

AU - Borca, Camelia

AU - Huthwelker, Thomas

AU - Jones, Davey L.

AU - Roose, Tiina

PY - 2022/4/1

Y1 - 2022/4/1

N2 - Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated.We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling.We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P.We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.

AB - Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated.We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling.We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P.We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.

KW - mycorrhizas

KW - plant phosphorus uptake

KW - rhizosphere modelling

KW - synchrotron

KW - X-ray computed tomography

KW - X-ray fluorescence

U2 - 10.1111/nph.17980

DO - 10.1111/nph.17980

M3 - Article

C2 - 35043984

VL - 234

SP - 688

EP - 703

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 2

ER -