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Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

Research output: Contribution to journal › Article › peer-review

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Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications. / Ongaro, Alfredo E; Di Giuseppe, Davide; Kermanizadeh, Ali et al.
In: Analytical Chemistry, Vol. 92, No. 9, 05.05.2020, p. 6693-6701.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Ongaro, AE, Di Giuseppe, D, Kermanizadeh, A, Miguelez Crespo, A, Mencattini, A, Ghibelli, L, Mancini, V, Wlodarczyk, KL, Hand, DP, Martinelli, E, Stone, V, Howarth, N, La Carrubba, V, Pensabene, V & Kersaudy-Kerhoas, M 2020, 'Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications', Analytical Chemistry, vol. 92, no. 9, pp. 6693-6701. https://doi.org/10.1021/acs.analchem.0c00651

APA

Ongaro, A. E., Di Giuseppe, D., Kermanizadeh, A., Miguelez Crespo, A., Mencattini, A., Ghibelli, L., Mancini, V., Wlodarczyk, K. L., Hand, D. P., Martinelli, E., Stone, V., Howarth, N., La Carrubba, V., Pensabene, V., & Kersaudy-Kerhoas, M. (2020). Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications. Analytical Chemistry, 92(9), 6693-6701. https://doi.org/10.1021/acs.analchem.0c00651

CBE

Ongaro AE, Di Giuseppe D, Kermanizadeh A, Miguelez Crespo A, Mencattini A, Ghibelli L, Mancini V, Wlodarczyk KL, Hand DP, Martinelli E, et al. 2020. Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications. Analytical Chemistry. 92(9):6693-6701. https://doi.org/10.1021/acs.analchem.0c00651

MLA

Ongaro, Alfredo E et al. "Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications". Analytical Chemistry. 2020, 92(9). 6693-6701. https://doi.org/10.1021/acs.analchem.0c00651

VancouverVancouver

Ongaro AE, Di Giuseppe D, Kermanizadeh A, Miguelez Crespo A, Mencattini A, Ghibelli L et al. Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications. Analytical Chemistry. 2020 May 5;92(9):6693-6701. Epub 2020 Apr 1. doi: 10.1021/acs.analchem.0c00651

Author

Ongaro, Alfredo E ; Di Giuseppe, Davide ; Kermanizadeh, Ali et al. / Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications. In: Analytical Chemistry. 2020 ; Vol. 92, No. 9. pp. 6693-6701.

RIS

TY - JOUR

T1 - Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

AU - Ongaro, Alfredo E

AU - Di Giuseppe, Davide

AU - Kermanizadeh, Ali

AU - Miguelez Crespo, Allende

AU - Mencattini, Arianna

AU - Ghibelli, Lina

AU - Mancini, Vanessa

AU - Wlodarczyk, Krystian L

AU - Hand, Duncan P

AU - Martinelli, Eugenio

AU - Stone, Vicki

AU - Howarth, Nicola

AU - La Carrubba, Vincenzo

AU - Pensabene, Virginia

AU - Kersaudy-Kerhoas, Maïwenn

PY - 2020/5/5

Y1 - 2020/5/5

N2 - Organ-on-chip (OOC) devices are miniaturized devices replacing animal models in drug discovery and toxicology studies. The majority of OOC devices are made from polydimethylsiloxane (PDMS), an elastomer widely used in microfluidic prototyping, but posing a number of challenges to experimentalists, including leaching of uncured oligomers and uncontrolled absorption of small compounds. Here we assess the suitability of polylactic acid (PLA) as a replacement material to PDMS for microfluidic cell culture and OOC applications. We changed the wettability of PLA substrates and demonstrated the functionalization method to be stable over a time period of at least 9 months. We successfully cultured human cells on PLA substrates and devices, without coating. We demonstrated that PLA does not absorb small molecules, is transparent (92% transparency), and has low autofluorescence. As a proof of concept of its manufacturability, biocompatibility, and transparency, we performed a cell tracking experiment of prostate cancer cells in a PLA device for advanced cell culture.

AB - Organ-on-chip (OOC) devices are miniaturized devices replacing animal models in drug discovery and toxicology studies. The majority of OOC devices are made from polydimethylsiloxane (PDMS), an elastomer widely used in microfluidic prototyping, but posing a number of challenges to experimentalists, including leaching of uncured oligomers and uncontrolled absorption of small compounds. Here we assess the suitability of polylactic acid (PLA) as a replacement material to PDMS for microfluidic cell culture and OOC applications. We changed the wettability of PLA substrates and demonstrated the functionalization method to be stable over a time period of at least 9 months. We successfully cultured human cells on PLA substrates and devices, without coating. We demonstrated that PLA does not absorb small molecules, is transparent (92% transparency), and has low autofluorescence. As a proof of concept of its manufacturability, biocompatibility, and transparency, we performed a cell tracking experiment of prostate cancer cells in a PLA device for advanced cell culture.

U2 - 10.1021/acs.analchem.0c00651

DO - 10.1021/acs.analchem.0c00651

M3 - Article

C2 - 32233401

VL - 92

SP - 6693

EP - 6701

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 9

ER -