TY - JOUR

T1 - Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot

AU - Yamada, Takatomi

AU - Mizuno, Ken-ichi

AU - Hirota, Kouji

AU - Kon, Ning

AU - Wahls, Wayne P

AU - Hartsuiker, Edgar

AU - Murofushi, Hiromu

AU - Shibata, Takehiko

AU - Ohta, Kunihiro

PY - 2004/4/21

Y1 - 2004/4/21

N2 - Histone acetyltransferases (HATs) and ATP-dependent chromatin remodeling factors (ADCRs) are involved in selective gene regulation via modulation of local chromatin configuration. Activation of the recombination hotspot ade6-M26 of Schizosaccharomyces pombe is mediated by a cAMP responsive element (CRE)-like sequence, M26, and a heterodimeric ATF/CREB transcription factor, Atf1.Pcr1. Chromatin remodeling occurs meiotically around M26. We examined the roles of HATs and ADCRs in chromatin remodeling around M26. Histones H3 and H4 around M26 were hyperacetylated in an M26- and Atf1-dependent manner early in meiosis. SpGcn5, the S. pombe homolog of Gcn5p, was required for the majority of histone H3 acetylation around M26 in vivo. Deletion of gcn5+ caused a significant delay in chromatin remodeling but only partial reduction of M26 meiotic recombination frequency. The snf22+ (a Swi2/Snf2-ADCR homologue) deletion and snf22+ gcn5+ double deletion abolished chromatin remodeling and significant reduction of meiotic recombination around M26. These results suggest that HATs and ADCRs cooperatively alter local chromatin structure, as in selective transcription activation, to activate meiotic recombination at M26 in a site-specific manner.

AB - Histone acetyltransferases (HATs) and ATP-dependent chromatin remodeling factors (ADCRs) are involved in selective gene regulation via modulation of local chromatin configuration. Activation of the recombination hotspot ade6-M26 of Schizosaccharomyces pombe is mediated by a cAMP responsive element (CRE)-like sequence, M26, and a heterodimeric ATF/CREB transcription factor, Atf1.Pcr1. Chromatin remodeling occurs meiotically around M26. We examined the roles of HATs and ADCRs in chromatin remodeling around M26. Histones H3 and H4 around M26 were hyperacetylated in an M26- and Atf1-dependent manner early in meiosis. SpGcn5, the S. pombe homolog of Gcn5p, was required for the majority of histone H3 acetylation around M26 in vivo. Deletion of gcn5+ caused a significant delay in chromatin remodeling but only partial reduction of M26 meiotic recombination frequency. The snf22+ (a Swi2/Snf2-ADCR homologue) deletion and snf22+ gcn5+ double deletion abolished chromatin remodeling and significant reduction of meiotic recombination around M26. These results suggest that HATs and ADCRs cooperatively alter local chromatin structure, as in selective transcription activation, to activate meiotic recombination at M26 in a site-specific manner.

KW - Acetylation

KW - Acetyltransferases

KW - Activating Transcription Factor 1

KW - Activating Transcription Factors

KW - Amino Acid Sequence

KW - Chromatin Assembly and Disassembly

KW - DNA-Binding Proteins

KW - Gene Deletion

KW - Genes, Fungal

KW - Histones

KW - Meiosis

KW - Molecular Sequence Data

KW - Phosphoproteins

KW - Recombination, Genetic

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Sequence Alignment

KW - Transcription Factors

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

U2 - 10.1038/sj.emboj.7600138

DO - 10.1038/sj.emboj.7600138

M3 - Article

C2 - 14988732

VL - 23

SP - 1792

EP - 1803

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 8

ER -