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Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling. / Ma, Qingxu; Kuzyakov, Yakov; Pan, Wankun et al.
In: The ISME Journal, Vol. 15, No. 11, 11.2021, p. 3148-3158.

Research output: Contribution to journalArticlepeer-review

HarvardHarvard

Ma, Q, Kuzyakov, Y, Pan, W, Tang, S, Chadwick, DR, Wen, Y, Hill, PW, Macdonald, A, Ge, T, Si, L, Wu, L & Jones, DL 2021, 'Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling', The ISME Journal, vol. 15, no. 11, pp. 3148-3158. https://doi.org/10.1038/s41396-021-00999-7

APA

Ma, Q., Kuzyakov, Y., Pan, W., Tang, S., Chadwick, D. R., Wen, Y., Hill, P. W., Macdonald, A., Ge, T., Si, L., Wu, L., & Jones, D. L. (2021). Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling. The ISME Journal, 15(11), 3148-3158. https://doi.org/10.1038/s41396-021-00999-7

CBE

Ma Q, Kuzyakov Y, Pan W, Tang S, Chadwick DR, Wen Y, Hill PW, Macdonald A, Ge T, Si L, et al. 2021. Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling. The ISME Journal. 15(11):3148-3158. https://doi.org/10.1038/s41396-021-00999-7

MLA

VancouverVancouver

Ma Q, Kuzyakov Y, Pan W, Tang S, Chadwick DR, Wen Y et al. Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling. The ISME Journal. 2021 Nov;15(11):3148-3158. Epub 2021 May 11. doi: 10.1038/s41396-021-00999-7

Author

Ma, Qingxu ; Kuzyakov, Yakov ; Pan, Wankun et al. / Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling. In: The ISME Journal. 2021 ; Vol. 15, No. 11. pp. 3148-3158.

RIS

TY - JOUR

T1 - Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling

AU - Ma, Qingxu

AU - Kuzyakov, Yakov

AU - Pan, Wankun

AU - Tang, Sheng

AU - Chadwick, David R.

AU - Wen, Yuan

AU - Hill, Paul W.

AU - Macdonald, Andy

AU - Ge, Tida

AU - Si, Linlin

AU - Wu, Lianghuan

AU - Jones, Davey L.

N1 - © 2021. The Author(s), under exclusive licence to International Society for Microbial Ecology.

PY - 2021/11

Y1 - 2021/11

N2 - Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied 13C, 15N, 14C, and 35S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO42− within hours. The SO42− was reutilised by the MB within days. The initial microbial utilisation and SO42− release were determined by amino acid structure. Met released 2.5-fold less SO42− than Cys. The microbial biomass retained comparatively more C and S from Met than Cys. The microorganisms decomposed Cys to pyruvate and H2S whereas they converted Met to α-ketobutyrate and S-CH3. The microbial stoichiometries of C, N, and S derived from Cys and Met were balanced after 4 d by Cys-derived SO42− uptake and Met-derived CO2 release. The microbial C:N:S ratio dynamics showed rapid C utilisation and loss, stable N levels, and S accumulation. Thus, short-term organic S utilisation by soil microorganisms is determined by amino acid structure whilst long-term organic S utilisation by soil microorganisms is determined by microbially controlled stoichiometry.

AB - Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied 13C, 15N, 14C, and 35S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO42− within hours. The SO42− was reutilised by the MB within days. The initial microbial utilisation and SO42− release were determined by amino acid structure. Met released 2.5-fold less SO42− than Cys. The microbial biomass retained comparatively more C and S from Met than Cys. The microorganisms decomposed Cys to pyruvate and H2S whereas they converted Met to α-ketobutyrate and S-CH3. The microbial stoichiometries of C, N, and S derived from Cys and Met were balanced after 4 d by Cys-derived SO42− uptake and Met-derived CO2 release. The microbial C:N:S ratio dynamics showed rapid C utilisation and loss, stable N levels, and S accumulation. Thus, short-term organic S utilisation by soil microorganisms is determined by amino acid structure whilst long-term organic S utilisation by soil microorganisms is determined by microbially controlled stoichiometry.

U2 - 10.1038/s41396-021-00999-7

DO - 10.1038/s41396-021-00999-7

M3 - Article

C2 - 33976391

VL - 15

SP - 3148

EP - 3158

JO - The ISME Journal

JF - The ISME Journal

SN - 1751-7370

IS - 11

ER -