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  • Katie N Myers
    University of Sheffield
  • Giancarlo Barone
    University of Sheffield
  • Anil Ganesh
    University of Sheffield
  • Christopher J Staples
    University of Sheffield
  • Anna E Howard
    University of Sheffield
  • Ryan D Beveridge
    University of Sheffield
  • Sarah Maslen
    Mass Spectrometry Group, Cambridge
  • J Mark Skehel
    Mass Spectrometry Group, Cambridge
  • Spencer J Collis
    University of Sheffield

It was recently discovered that vertebrate genomes contain multiple endogenised nucleotide sequences derived from the non-retroviral RNA bornavirus. Strikingly, some of these elements have been evolutionary maintained as open reading frames in host genomes for over 40 million years, suggesting that some endogenised bornavirus-derived elements (EBL) might encode functional proteins. EBLN1 is one such element established through endogenisation of the bornavirus N gene (BDV N). Here, we functionally characterise human EBLN1 as a novel regulator of genome stability. Cells depleted of human EBLN1 accumulate DNA damage both under non-stressed conditions and following exogenously induced DNA damage. EBLN1-depleted cells also exhibit cell cycle abnormalities and defects in microtubule organisation as well as premature centrosome splitting, which we attribute in part, to improper localisation of the nuclear envelope protein TPR. Our data therefore reveal that human EBLN1 possesses important cellular functions within human cells, and suggest that other EBLs present within vertebrate genomes may also possess important cellular functions.

Original languageEnglish
Pages (from-to)35548
JournalScientific Reports
Publication statusPublished - 14 Oct 2016

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