TY - JOUR

T1 - The effect of severe eccentric exercise-induced muscle damage on plasma elastase, glutamine and zinc concentrations

AU - Gleeson, M

AU - Walsh, Neil

AU - Blannin, Andrew K.

AU - Robson, P. J.

AU - Cook, L.

AU - Donnelly, A.E.

AU - Day, S.H.

PY - 1998/5

Y1 - 1998/5

N2 - The aim of this study was to determine if severe exercise-induced muscle damage alters the plasma concentrations of glutamine and zinc. Changes in plasma concentrations of glutamine, zinc and polymorphonuclear elastase (an index of phagocytic cell activation) were examined for up to 10 days following eccentric exercise of the knee extensors of one leg in eight untrained subjects. The exercise bout consisted of 20 repetitions of electrically stimulated eccentric muscle actions on an isokinetic dynamometer. Subjects experienced severe muscle soreness and large increases in plasma creatine kinase activity indicative of muscle fibre damage. Peak soreness occurred at 2 days post-exercise and peak creatine kinase activity [21714 (6416) U x l(-1) mean (SEM)] occurred at 3 days post-exercise (P < 0.01 compared with pre-exercise). Plasma elastase concentration was increased at 3 days post-exercise compared with pre-exercise (P < 0.05), and is presumably indicative of ongoing phagocytic leucocyte infiltration and activation in the damaged muscles. There were no significant changes in plasma zinc and glutamine concentrations in the days following eccentric exercise. We conclude that exercise-induced muscle damage does not produce changes in plasma glutamine or zinc concentrations despite evidence of phagocytic neutrophil activation.

AB - The aim of this study was to determine if severe exercise-induced muscle damage alters the plasma concentrations of glutamine and zinc. Changes in plasma concentrations of glutamine, zinc and polymorphonuclear elastase (an index of phagocytic cell activation) were examined for up to 10 days following eccentric exercise of the knee extensors of one leg in eight untrained subjects. The exercise bout consisted of 20 repetitions of electrically stimulated eccentric muscle actions on an isokinetic dynamometer. Subjects experienced severe muscle soreness and large increases in plasma creatine kinase activity indicative of muscle fibre damage. Peak soreness occurred at 2 days post-exercise and peak creatine kinase activity [21714 (6416) U x l(-1) mean (SEM)] occurred at 3 days post-exercise (P < 0.01 compared with pre-exercise). Plasma elastase concentration was increased at 3 days post-exercise compared with pre-exercise (P < 0.05), and is presumably indicative of ongoing phagocytic leucocyte infiltration and activation in the damaged muscles. There were no significant changes in plasma zinc and glutamine concentrations in the days following eccentric exercise. We conclude that exercise-induced muscle damage does not produce changes in plasma glutamine or zinc concentrations despite evidence of phagocytic neutrophil activation.

KW - Adult

KW - Cell Degranulation

KW - Exercise

KW - Female

KW - Glutamine

KW - Humans

KW - Leukocyte Elastase

KW - Male

KW - Muscle, Skeletal

KW - Neutrophils

KW - Phagocytosis

KW - Zinc

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s004210050373

DO - 10.1007/s004210050373

M3 - Article

C2 - 9650740

VL - 77

SP - 543

EP - 546

JO - European journal of applied physiology and occupational physiology

JF - European journal of applied physiology and occupational physiology

SN - 1439-6319

IS - 6

ER -