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  • Andrew Oliphant
    Aberystwyth UniversityInstitute of Biological, Environmental & Rural Sciences (IBERS)
  • Jodi L. Alexander
    School of Biological Sciences, Bangor University, Bangor, Gwynedd, Wales, United Kingdom.
  • Martin T. Swain
    Institute of Biological, Environmental & Rural Sciences (IBERS)
  • Simon G. Webster
  • David C. Wilcockson
    Institute of Biological, Environmental & Rural Sciences (IBERS)
Abstract
Background: Ecdysis is an innate behaviour programme by which all arthropods moult their exoskeletons. The
complex suite of interacting neuropeptides that orchestrate ecdysis is well studied in insects, but details of the
crustacean ecdysis cassette are fragmented and our understanding of this process is comparatively crude,
preventing a meaningful evolutionary comparison. To begin to address this issue we identified transcripts coding
for neuropeptides and their putative receptors in the central nervous system (CNS) and Y-organs (YO) within the
crab, Carcinus maenas, and mapped their expression profiles across accurately defined stages of the moult cycle
using RNA-sequencing. We also studied gene expression within the epidermally-derived YO, the only defined role
for which is the synthesis of ecdysteroid moulting hormones, to elucidate peptides and G protein-coupled
receptors (GPCRs) that might have a function in ecdysis.
Results: Transcriptome mining of the CNS transcriptome yielded neuropeptide transcripts representing 47
neuropeptide families and 66 putative GPCRs. Neuropeptide transcripts that were differentially expressed across the
moult cycle included carcikinin, crustacean hyperglycemic hormone-2, and crustacean cardioactive peptide, whilst a
single putative neuropeptide receptor, proctolin R1, was differentially expressed. Carcikinin mRNA in particular
exhibited dramatic increases in expression pre-moult, suggesting a role in ecdysis regulation. Crustacean
hyperglycemic hormone-2 mRNA expression was elevated post- and pre-moult whilst that for crustacean
cardioactive peptide, which regulates insect ecdysis and plays a role in stereotyped motor activity during
crustacean ecdysis, was elevated in pre-moult.
In the YO, several putative neuropeptide receptor transcripts were differentially expressed across the moult cycle, as
was the mRNA for the neuropeptide, neuroparsin-1. Whilst differential gene expression of putative neuropeptide
receptors was expected, the discovery and differential expression of neuropeptide transcripts was surprising.
Analysis of GPCR transcript expression between YO and epidermis revealed 11 to be upregulated in the YO and
thus are now candidates for peptide control of ecdysis.
Conclusions: The data presented represent a comprehensive survey of the deduced C. maenas neuropeptidome
and putative GPCRs. Importantly, we have described the differential expression profiles of these transcripts across
accurately staged moult cycles in tissues key to the ecdysis programme. This study provides important avenues for
the future exploration of functionality of receptor-ligand pairs in crustaceans.
Keywords: Ecdysis, G protein-coupled receptor, Central nervous system, Y-organ

Keywords

  • Ecdysis, G protein-coupled receptor, Central nervous system, Y-organ
Original languageEnglish
Article number19:711
Number of pages26
JournalBMC Genomics
Volume19
Issue number711
DOIs
Publication statusPublished - 26 Sep 2018

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