This paper introduces the first results of dielectric spectroscopy characterization of glioblastoma cells, measuring their crossover frequencies in the ultra-high-frequency range (above 50 MHz) by dielectrophoresis (DEP) techniques. Experiments were performed on two glioblastoma lines U87-MG and LN18 that were cultured following different conditions, in order to achieve different phenotypic profiles. We demonstrate here that the presented DEP electrokinetic method can be used to discriminate the undifferentiated from the differentiated cells. In this study, microfluidic lab-on-chip systemsimplementedonbipolarcomplementaryoxidesemiconductortechnologyareusedallowing single cell handling and analysis. Based on the characterizations of their own intracellular features, both the selected glioblastoma (GBMcelllinesculturedindistinctcultureconditionshaveshown cleardifferencesofDEPcrossoverfrequencysignaturescompared to the differentiated cells cultured in a normal medium. These results support the concept and validate the efficiency for cell characterization in glioblastoma pathology