Venom-on-a-chip: a fast and efficient method for comparative venomics

Research output: Contribution to journalArticle

Standard Standard

Venom-on-a-chip: a fast and efficient method for comparative venomics. / Zancolli, Giulia; Sanz, Libia; Calvete, Juan J.; Wuster, Wolfgang.

In: Toxins, Vol. 9, No. 179, 28.05.2017, p. 1-15.

Research output: Contribution to journalArticle

HarvardHarvard

Zancolli, G, Sanz, L, Calvete, JJ & Wuster, W 2017, 'Venom-on-a-chip: a fast and efficient method for comparative venomics', Toxins, vol. 9, no. 179, pp. 1-15.

APA

Zancolli, G., Sanz, L., Calvete, J. J., & Wuster, W. (2017). Venom-on-a-chip: a fast and efficient method for comparative venomics. Toxins, 9(179), 1-15.

CBE

Zancolli G, Sanz L, Calvete JJ, Wuster W. 2017. Venom-on-a-chip: a fast and efficient method for comparative venomics. Toxins. 9(179):1-15.

MLA

Zancolli, Giulia et al. "Venom-on-a-chip: a fast and efficient method for comparative venomics". Toxins. 2017, 9(179). 1-15.

VancouverVancouver

Zancolli G, Sanz L, Calvete JJ, Wuster W. Venom-on-a-chip: a fast and efficient method for comparative venomics. Toxins. 2017 May 28;9(179):1-15.

Author

Zancolli, Giulia ; Sanz, Libia ; Calvete, Juan J. ; Wuster, Wolfgang. / Venom-on-a-chip: a fast and efficient method for comparative venomics. In: Toxins. 2017 ; Vol. 9, No. 179. pp. 1-15.

RIS

TY - JOUR

T1 - Venom-on-a-chip: a fast and efficient method for comparative venomics

AU - Zancolli, Giulia

AU - Sanz, Libia

AU - Calvete, Juan J.

AU - Wuster, Wolfgang

PY - 2017/5/28

Y1 - 2017/5/28

N2 - Venom research has attracted an increasing interest in disparate fields, from drugdevelopment and pharmacology, to evolutionary biology and ecology, and rational antivenomproduction. Advances in “-omics” technologies have allowed the characterization of an increasingnumber of animal venoms, but the methodology currently available is suboptimal for large-scalecomparisons of venom profiles. Here, we describe a fast, reproducible and semi-automated protocolfor investigating snake venom variability, especially at the intraspecific level, using the AgilentBioanalyzer on-chip technology. Our protocol generated a phenotype matrix which can be usedfor robust statistical analysis and correlations of venom variation with ecological correlates, orother extrinsic factors. We also demonstrate the ease and utility of combining on-chip technologywith previously fractionated venoms for detection of specific individual toxin proteins. Our studydescribes a novel strategy for rapid venom discrimination and analysis of compositional variation atmultiple taxonomic levels, allowing researchers to tackle evolutionary questions and unveiling thedrivers of the incredible biodiversity of venoms.

AB - Venom research has attracted an increasing interest in disparate fields, from drugdevelopment and pharmacology, to evolutionary biology and ecology, and rational antivenomproduction. Advances in “-omics” technologies have allowed the characterization of an increasingnumber of animal venoms, but the methodology currently available is suboptimal for large-scalecomparisons of venom profiles. Here, we describe a fast, reproducible and semi-automated protocolfor investigating snake venom variability, especially at the intraspecific level, using the AgilentBioanalyzer on-chip technology. Our protocol generated a phenotype matrix which can be usedfor robust statistical analysis and correlations of venom variation with ecological correlates, orother extrinsic factors. We also demonstrate the ease and utility of combining on-chip technologywith previously fractionated venoms for detection of specific individual toxin proteins. Our studydescribes a novel strategy for rapid venom discrimination and analysis of compositional variation atmultiple taxonomic levels, allowing researchers to tackle evolutionary questions and unveiling thedrivers of the incredible biodiversity of venoms.

KW - Bioanalyzer

KW - Crotalus

KW - on-chip electrophoresis

KW - population-level variation

KW - venom composition

UR - http://www.mdpi.com/2072-6651/9/6/179

M3 - Article

VL - 9

SP - 1

EP - 15

JO - Toxins

JF - Toxins

SN - 2072-6651

IS - 179

ER -