Professor Alexander Yakunin
Professor In Enzyme Discovery and Application

Contact info
Professor Alexander Yakunin (Iakounine)
Office: Thoday Building, room S25
Email: a.iakounine@bangor.ac.uk
Tel: 01248 388399
Research focus: enzyme discovery, enzymology, enzyme applications
- Erthygl › Ymchwil › Adolygwyd gan gymheiriaid
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A family of metal-dependent phosphatases implicated in metabolite damage-control
Huang, L., Khusnutdinova, A., Nocek, B., Brown, G., Xu, X., Cui, H., Petit, P., Flick, R., Zallot, R., Balmant, K., Ziemak, M. J., Shanklin, J., de Crecy-Lagard, V., Fiehn, O., Gregory, J. F. ,. I., Joachimiak, A., Savchenko, A., Yakunin, A. F. & Hanson, A. D., Awst 2016, Yn: Nature Chemical Biology. 12, 8, t. 621-627Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
- Cyhoeddwyd
A microplate screen to estimate metal-binding affinities of metalloproteins
Diep, P., Mahadevan, R. & Yakunin, A. F., 15 Tach 2020, Yn: Analytical biochemistry. 609, t. 113836Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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A random mutagenesis screen enriched for missense mutations in bacterial effector proteins
Urbanus, M. L., Zheng, T. M., Khusnutdinova, A. N., Banh, D., O'Connor Mount, H., Gupta, A., Stogios, P. J., Savchenko, A., Isberg, R. R., Yakunin, A. F. & Ensminger, A. W., 4 Medi 2024, Yn: G3: Genes, Genomes, Genetics. 14, 9Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families
Popovic, A., Hai, T., Tchigvintsev, A., Hajighasemi, M., Nocek, B., Khusnutdinova, A. N., Brown, G., Glinos, J., Flick, R., Skarina, T., Chernikova, T., Yim, V., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Ferrer, M., Golyshina, O., Savchenko, A., Golyshin, P. & Yakunin, A. F., Maw 2017, Yn: Scientific Reports. 7, 44103, 44103.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid
Joo, J. C., Khusnutdinova, A. N., Flick, R., Kim, T., Bornscheuer, U. T., Yakunin, A. F. & Mahadevan, R., 1 Chwef 2017, Yn: Chemical Science. 8, 2, t. 1406-1413Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
- Cyhoeddwyd
An Amino Acid Self-Regenerating Cell-Free Protein Synthesis System that Feeds on PLA Plastics, CO2, Ammonium, and α-ketoglutarate
Nishikawa, S., Yu, W.-C., Jia, T. Z., He, M.-J., Khusnutdinova, A., Yakunin, A., Chiang, Y.-R., Fujishima, K. & Wang, P.-H., 17 Mai 2024, Yn: ACS Catalysis. 14, 10, t. 7696-7706Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
- Cyhoeddwyd
Biochemical and structural insights into enzymatic depolymerization of polylactic acid and other polyesters by microbial carboxylesterases
Hajighasemi, M., Nocek, B. P., Tchigvintsev, A., Brown, G., Flick, R., Xu, X., Cui, H., Hai, T., Joachimiak, A., Golyshin, P., Savchenko, A., Edwards, E. A. & Yakunin, A. F., 18 Ebr 2016, Yn: Biomacromolecules. 17, 6, t. 2027-2039Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
- Cyhoeddwyd
Biochemical plasticity of the Escherichia coli CRISPR Cascade revealed by in vitro reconstitution of Cascade activities from purified Cas proteins
Lemak, S., Brown, G., Makarova, K. S., Koonin, E. V. & Yakunin, A., 2024, Yn: Febs Journal. t. 1-18 18 t.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Biosynthesis and activity of prenylated FMN cofactors
Wang, P.-H., Khusnutdinova, A. N., Luo, F., Xiao, J., Nemr, K., Flick, R., Brown, G., Mahadevan, R., Edwards, E. & Yakunin, A., 17 Mai 2018, Yn: Cell Chemical Biology. 25, 5, t. 560-570Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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CRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli
Beloglazova, N., Kuznedelov, K., Flick, R., Datsenko, K. A., Brown, G., Popovic, A., Lemak, S., Semenova, E., Severinov, K. & Yakunin, A. F., Ion 2015, Yn: Nucleic Acids Research. 43, 1, t. 530-543Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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CRISPR-induced DNA reorganization for multiplexed nucleic acid detection
Karlikow, M., Amalfitano, E., Yang, X., Doucet, J., Chapman, A., Sadat-Moussavi, P., Homme, P., Sutyrina, P., Chang, W., Lemak, S., Yakunin, A., Dolezal, A. G., Kelley, S., Foster, L., Harpur, B. & Pardee, K., 17 Maw 2023, Yn: Nature Communications. 14, 1, 1505.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Determinants and prediction of esterase substrate promiscuity patterns
Martínez-Martínez, M., Coscolín, C., Santiago, G., Chow, J., Stogios, P. J., Bargiela, R., Gertler, C., Navarro-Fernández, J., Bollinger, A., Thies, S., Méndez-García, C., Popovic, A., Brown, G., Chernikova, T., García-Moyano, A., Bjerga, G. E. K., Perez-Garcia, P., Hai, T., del Pozo, M. V., Stokke, R., Steen, I. H., Cui, H., Xu, X., Nocek, B., Alcaide, M., Disasto, M., Mesa, V., Pelaez, A. I., Sanchez, J., Buchholz, P. C. F., Pleiss, J., Fernández-Guerra, A., Glockner, F. O., Golyshina, O., Yakimov, M. M., Savchenko, A., Jaeger, K.-E., Yakunin, A. F., Streit, W. R., Golyshin, P., Guallar, V. & Ferrer, M., 2018, Yn: ACS Chemical Biology. 13, 1, t. 225-234Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase
Placido, A., Hai, T., Ferrer, M., Chernikova, T. N., Distaso, M. A., Armstrong, D., Yakunin, A. F., Toshchakov, S. V., Yakimov, M. M., Kublanov, I. V., Golyshina, O., Pesole, G., Ceci, L. R. & Golyshin, P., 1 Rhag 2015, Yn: Applied Microbiology and Biotechnology. 99, 23, t. 10031-10046Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Engineering a short, aldolase based pathway for (R)-1,3-butanediol production in Escherichia coli
Nemr, K., Muller, J., Joo, J. C., Gawand, P., Cjoudhary, R., Mendonca, B., Lu, S., Yu, X., Yakunin, A. & Mahadevan, R., 1 Gorff 2018, Yn: Metabolic Engineering. 48, t. 13-24Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
Golyshina, O., Hai, T., Kube, M., Chernikova, T. N., Al-Ramahi, Y., Beloqui, A., Lopez-Cortez, N., Guazzaroni, M. E., Heipieper, H. J., Klages, S., Kotsyurbenko, O. R., Langer, I., Nechitaylo, T. Y., Lünsdorf, H., Fernández, M., Juárez, S., Ciordia, S., Singer, A., Kagan, O., Egorova, O., Petit, P. A., Stogios, P., Kim, Y., Tchigvintsev, A., Flick, R., Denaro, R., Genovese, M., Albar, J. P., Reva, O. N., Martínez-Gomariz, M., Tran, H., Ferrer, M., Savchenko, A., Yakunin, A. F., Yakimov, M. M., Golyshina, O. V., Reinhardt, R. & Golyshin, P. N., 23 Gorff 2013, Yn: Nature Communications. 4, t. 2156Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Heavy metal removal by bioaccumulation using genetically engineered microorganisms
Diep, P., Mahadevan, R. & Yakunin, A., 29 Hyd 2018, Yn: Frontiers in Bioengineering and Biotechnology. 6, 157.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Identification and characterization of carboxyl esterases of gill chamber-associated microbiota in the deep-sea shrimp Rimicaris exoculata using functional metagenomics
Alcaide, M., Tchigvinstsev, A., Martinez-Martinez, M., Popovic, A., Reva, O. N., Lafraya, A., Bargiela, R. N., Nechitaylo, T. Y., Matesanz, R., Cambon-Bonavita, M., Jebbar, M., Savchenko, A., Yakimov, M. M., Golyshina, O., Yakunin, A. F., Golyshin, P., Ferrer, M. & The MAMBA Consortium., [. V., 16 Ion 2015, Yn: Applied and Environmental Microbiology. 81, 6, t. 2125-2136Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin
Choolaei, Z., Flick, R., Khusnutdinova, A., Edwards, E. A. & Yakunin, A. F., 10 Ion 2021, Yn: Journal of Biotechnology. 325, t. 128-137 10 t.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT
Golyshina, O., Hai, T., Reva, O. N., Lemak, S., Yakunin, A. F., Goesmann, A., Nechitaylo, T. Y., LaCono, V., Smedile, F., Slesarev, A., Rojo, D., Barbas, C., Ferrer, M., Yakimov, M. M. & Golyshin, P., 16 Meh 2017, Yn: Scientific Reports. 7, 3682.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Metabolite Damage and Damage-Control in a Minimal Genome
Haas, D., Thamm, A. M., Sun, J., Huang, L., Sun, L., Beaudoin, G. A. W., Wise, K. S., Lema-Ortiz, C., Bruner, S. D., Breuer, M., Luthey-Schutten, Z., Lin, J., Wilson, M. A., Brown, G., Yakunin, A., Kurilyak, I., Folz, J., Fiehn, O., Glass, J. I., Hanson, A. D., Henry, C. S. & de Crecy-Lagard, V., 30 Awst 2022, Yn: mBio. e0163022.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5
Khusnutdinova, A., Hai, T., Devlekar, S., Distaso, M., Kublanov, I. V., Skarina, T., Stogios, P., Savchenko, A., Ferrer, M., Golyshina, O., Yakunin, A. & Golyshin, P., Medi 2024, Yn: Fems Microbiology Ecology. 100, 9, fiae114.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Ni(II)-binding affinity of CcNikZ-II and its homologs: the role of the HH-prong and variable loop revealed by structural and mutational studies
Diep, P., Stogios, P. J., Evdokimova, E., Savchenko, A., Mahadevan, R. & Yakunin, A. F., Gorff 2024, Yn: Febs Journal. 291, 13, t. 2980-2993 14 t.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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One-pot biocatalytic transformation of adipic acid to 6-aminocaproic acid and 1,6-hexamethylenediamine using carboxylic acid reductases and transaminases
Fedorchuk, T. P., Khusnutdinova, A. N., Evdokimova, E., Flick, R., Di Leo, R., Stogios, P. J., Savchenko, A. & Yakunin, A., 15 Ion 2020, Yn: Journal of the American Chemical Society. 142, 2, t. 1038-1048 11 t.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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One-pot chemo-enzymatic synthesis and one-step recovery of length-variable long-chain polyphosphates from microalgal biomass
Lin, Y.-H., Nishikawa, S., Jia, T. Z., Yeh, F.-I., Khusnutdinova, A., Yakunin, A., Fujishima, K. & Wang, P.-H., 27 Hyd 2023, Yn: Green Chemistry. 23Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Polyesterase activity is widespread in the family IV carboxylesterases from bacteria
Ma, H., Khusnutdinova, A. N., Lemak, S., Chernikova, T. N., Golyshina, O. V., Almendral, D., Ferrer, M., Golyshin, P. N. & Yakunin, A. F., 5 Ion 2025, Yn: Journal of Hazardous Materials. 481, t. 136540Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid