A GAL4-Driver Line Resource for Drosophila Neurobiology
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Yn: Cell Reports, Cyfrol 2, Rhif 4, 11.10.2012, t. 991-1001.
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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T1 - A GAL4-Driver Line Resource for Drosophila Neurobiology
AU - Jenett, Arnim
AU - Rubin, Gerald
AU - Ngo, Teri T.B.
AU - Shepherd, David
AU - Murphy, Christine
AU - Dionne, Heather
AU - Pfeiffer, Barrett
AU - Cavallaro, Amanda
AU - Hall, Donald
AU - Jeter, Jennifer
AU - Iyer, Nirmal
AU - Fetter, Dona
AU - Hausenfluck, Joanna
AU - Peng, Hanchuan
AU - Trautman, Eric T.
AU - Svirskas, Robert R.
AU - Meyers, Eugene W.
AU - Iwinski, Zbigniew R.
AU - Aso, Yoshnori
AU - DePasquale, Gina M.
AU - Enos, Adrianne
AU - Hulamm, Phuson
AU - Lam, Shing Chun Benny
AU - Li, Hsing-Hsi
AU - Laverty, Todd
AU - Long, Fuhui
AU - Qu, Lei
AU - Murphy, Sean D.
AU - Rokicki, Conrad
AU - Safford, Todd
AU - Shaw, Kshiti
AU - Simpson, Julie H.
AU - Sowell, Allison
AU - Tae, Susana
AU - Yu, Yang
AU - Zugates, Christopher T.
PY - 2012/10/11
Y1 - 2012/10/11
N2 - We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
AB - We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
U2 - 10.1016/j.celrep.2012.09.011
DO - 10.1016/j.celrep.2012.09.011
M3 - Article
VL - 2
SP - 991
EP - 1001
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 4
ER -