TY - JOUR

T1 - Black phosphorus based fiber optic biosensor for ultrasensitive cancer diagnosis

AU - Zhou, Lin

AU - Liu, Chen

AU - Sun, Zhengbo

AU - Mao, Hongju

AU - Zhang, Lin

AU - Yu, Xuefeng

AU - Zhao, Jianlong

AU - Chen, Xianfeng

N1 - Supplementary data related to this article can be found at https://doi.org/10.1016/j.bios.2019.04.044. The authors acknowledge support from the projects of EU FP7 PIRSES-GA-2013-612267 (iPhoto Bio, European Commission); the Sêr Cymru NRN Fund (NRN097, United Kingdom); the National Natural Science Foundation of China (No. 61571429 and 61801464, China).

PY - 2019/7/15

Y1 - 2019/7/15

N2 - We propose the first black phosphorus (BP) – fiber optic biosensor for ultrasensitive diagnosis of human neuronspecific enolase (NSE) cancer biomarkers. A novel optical-nano configuration has been exploited by integrating BP nanosheets with a largely tilted fiber grating (BP-TFG), where the BP is bio-functionalized by the poly-Llysine acting as a critical cross-linker to facilitate bio-nano-photonic interface with extremely enhanced lightmatter interaction. BP nanosheets are synthesized by a liquid ultrasonication-based exfoliation and deposited on fiber device by an in-situ layer-by-layer method. The BP-induced optical modulation effects in terms of thicknesstunable feature, polarization-dependence and enhanced light-matter interaction are experimentally investigated. The anti-NSE immobilized BP-TFG biosensor has been implemented to detect NSE biomarkers demonstratingultrahigh sensitivity with limit of detection down to 1.0 pg/mL, which is 4 orders magnitude lower than NSE cutoff value of small cell lung cancer. The enhanced sensitivity of BP-TFG is 100-fold higher than graphene oxide or AuNPs based biosensors. We believe that BP-fiber optic configuration opens a new bio-nano-photonic platform for the applications in healthcare, biomedical, food safety and environmental monitoring.

AB - We propose the first black phosphorus (BP) – fiber optic biosensor for ultrasensitive diagnosis of human neuronspecific enolase (NSE) cancer biomarkers. A novel optical-nano configuration has been exploited by integrating BP nanosheets with a largely tilted fiber grating (BP-TFG), where the BP is bio-functionalized by the poly-Llysine acting as a critical cross-linker to facilitate bio-nano-photonic interface with extremely enhanced lightmatter interaction. BP nanosheets are synthesized by a liquid ultrasonication-based exfoliation and deposited on fiber device by an in-situ layer-by-layer method. The BP-induced optical modulation effects in terms of thicknesstunable feature, polarization-dependence and enhanced light-matter interaction are experimentally investigated. The anti-NSE immobilized BP-TFG biosensor has been implemented to detect NSE biomarkers demonstratingultrahigh sensitivity with limit of detection down to 1.0 pg/mL, which is 4 orders magnitude lower than NSE cutoff value of small cell lung cancer. The enhanced sensitivity of BP-TFG is 100-fold higher than graphene oxide or AuNPs based biosensors. We believe that BP-fiber optic configuration opens a new bio-nano-photonic platform for the applications in healthcare, biomedical, food safety and environmental monitoring.

U2 - 10.1016/j.bios.2019.04.044

DO - 10.1016/j.bios.2019.04.044

M3 - Article

VL - 137

SP - 140

EP - 147

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -