Fersiynau electronig


Dangosydd eitem ddigidol (DOI)

  • Warish Ahmed
    CSIRO Land and Water, Canberra
  • Paul Bertsch
    CSIRO Land and Water, Canberra
  • Aaron Bivins
    University of Notre Dame, Indiana
  • Kyle Bibby
    University of Notre Dame, Indiana
  • Kata Farkas
  • Amy Gathercole
    South Australian Health and Medical Research Institute
  • Eiji Haramoto
    University of Yamanashi
  • Pradip Gyawali
    Institute of Environmental Science and Research, NZ
  • Asja Korajkic
    United States Environmental Protection Agency
  • Brian McMinn
    United States Environmental Protection Agency
  • Jochen Mueller
    University of Queensland
  • Stuart Simpson
    CSIRO Land and Water, Canberra
  • Wendy Smith
    CSIRO Agriculture and Food
  • Erin Symonds
    University of South Florida
  • Kevin Thomas
    University of Queensland
  • Rory Verhagen
    University of Queensland
  • Masaaki Kitajima
    Hokkaido University
There is currently a clear benefit for many countries to utilize wastewater-based epidemiology (WBE) as part of ongoing measures to manage the coronavirus disease 2019 (COVID-19) global pandemic. Since most wastewater virus concentration methods were developed and validated for nonenveloped viruses, it is imperative to determine the efficiency of the most commonly used methods for the enveloped severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Municipal wastewater seeded with a human coronavirus (CoV) surrogate, murine hepatitis virus (MHV), was used to test the efficiency of seven wastewater virus concentration methods: (A–C) adsorption-extraction with three different pre-treatment options, (D–E) centrifugal filter device methods with two different devices, (F) polyethylene glycol (PEG 8000) precipitation, and (G) ultracentrifugation. MHV was quantified by reverse-transcription quantitative polymerase chain reaction and the recovery efficiency was calculated for each method. The mean MHV recoveries ranged from 26.7 to 65.7%. The most efficient methods were adsorption-extraction methods with MgCl2 pre-treatment (Method C), and without pre-treatment (Method B). The third most efficient method used the Amicon® Ultra-15 centrifugal filter device (Method D) and its recovery efficiency was not statistically different from the most efficient methods. The methods with the worst recovery efficiency included the adsorption-extraction method with acidification (A), followed by PEG precipitation (F). Our results suggest that absorption-extraction methods with minimal or without pre-treatment can provide suitably rapid, cost-effective and relatively straightforward recovery of enveloped viruses in wastewater. The MHV is a promising process control for SARS-CoV-2 surveillance and can be used as a quality control measure to support community-level epidemic mitigation and risk assessment.
Iaith wreiddiolSaesneg
Rhif yr erthygl139960
CyfnodolynScience of the Total Environment
Dyddiad ar-lein cynnar5 Meh 2020
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 15 Hyd 2020

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