Declining reactivation ability of Escherichia coli O157 following incubation within soil

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Declining reactivation ability of Escherichia coli O157 following incubation within soil. / Williams, A.P.; Gordon, H.E.; Jones, D.L. et al.
Yn: Soil Biology and Biochemistry, Cyfrol 63, 01.08.2013, t. 85-88.

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

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Williams, AP, Gordon, HE, Jones, DL, Killham, K, Strachan, NJ & Forbes, KJ 2013, 'Declining reactivation ability of Escherichia coli O157 following incubation within soil', Soil Biology and Biochemistry, cyfrol. 63, tt. 85-88. https://doi.org/10.1016/j.soilbio.2013.03.031

APA

Williams, A. P., Gordon, H. E., Jones, D. L., Killham, K., Strachan, N. J., & Forbes, K. J. (2013). Declining reactivation ability of Escherichia coli O157 following incubation within soil. Soil Biology and Biochemistry, 63, 85-88. https://doi.org/10.1016/j.soilbio.2013.03.031

CBE

MLA

VancouverVancouver

Williams AP, Gordon HE, Jones DL, Killham K, Strachan NJ, Forbes KJ. Declining reactivation ability of Escherichia coli O157 following incubation within soil. Soil Biology and Biochemistry. 2013 Awst 1;63:85-88. doi: 10.1016/j.soilbio.2013.03.031

Author

Williams, A.P. ; Gordon, H.E. ; Jones, D.L. et al. / Declining reactivation ability of Escherichia coli O157 following incubation within soil. Yn: Soil Biology and Biochemistry. 2013 ; Cyfrol 63. tt. 85-88.

RIS

TY - JOUR

T1 - Declining reactivation ability of Escherichia coli O157 following incubation within soil

AU - Williams, A.P.

AU - Gordon, H.E.

AU - Jones, D.L.

AU - Killham, K.

AU - Strachan, N.J.

AU - Forbes, K.J.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Soil is known to play an important role in the cycling of the human pathogen Escherichia coli O157 in the environment. Here we investigated whether residence time in soil influenced its ability to not only survive but subsequently reactivate after release into water during a simulated rainfall event. We inoculated an agricultural soil with chromosomally lux-marked E. coli O157 and then incubated it at 4 or 15 C for up to 120 d, at which point the pathogen was recovered by extracting the soil with rainwater and its reactivation measured by monitoring bioluminescence over a 9 h period. We found that with increasing residence time in soil, E. coli O157 exhibited a reduction in both numbers and reactivation capacity as measured by cells; energy status (bioluminescence). As the degree of cell activity is linked to infectivity, this indicates that transmissibility may be reduced after a period within soil. This should be considered when assessing the degree of risk from environmental exposure to bacterial pathogens.

AB - Soil is known to play an important role in the cycling of the human pathogen Escherichia coli O157 in the environment. Here we investigated whether residence time in soil influenced its ability to not only survive but subsequently reactivate after release into water during a simulated rainfall event. We inoculated an agricultural soil with chromosomally lux-marked E. coli O157 and then incubated it at 4 or 15 C for up to 120 d, at which point the pathogen was recovered by extracting the soil with rainwater and its reactivation measured by monitoring bioluminescence over a 9 h period. We found that with increasing residence time in soil, E. coli O157 exhibited a reduction in both numbers and reactivation capacity as measured by cells; energy status (bioluminescence). As the degree of cell activity is linked to infectivity, this indicates that transmissibility may be reduced after a period within soil. This should be considered when assessing the degree of risk from environmental exposure to bacterial pathogens.

U2 - 10.1016/j.soilbio.2013.03.031

DO - 10.1016/j.soilbio.2013.03.031

M3 - Article

VL - 63

SP - 85

EP - 88

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -