Determination of poly-β-hydroxyalkanoate in Peat
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Yn: Communications in Soil Science and Plant Analysis, Cyfrol 48, Rhif 5, 02.2017, t. 576-580.
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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TY - JOUR
T1 - Determination of poly-β-hydroxyalkanoate in Peat
AU - Dowrick, David
AU - Yusoff, Fatimah
AU - Gough, Rachel
AU - Freeman, Christopher
PY - 2017/2
Y1 - 2017/2
N2 - Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.
AB - Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.
U2 - 10.1080/00103624.2017.1282506
DO - 10.1080/00103624.2017.1282506
M3 - Article
VL - 48
SP - 576
EP - 580
JO - Communications in Soil Science and Plant Analysis
JF - Communications in Soil Science and Plant Analysis
SN - 0010-3624
IS - 5
ER -