TY - JOUR

T1 - Determination of poly-β-hydroxyalkanoate in Peat

AU - Dowrick, David

AU - Yusoff, Fatimah

AU - Gough, Rachel

AU - Freeman, Christopher

PY - 2017/2

Y1 - 2017/2

N2 - Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.

AB - Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material which has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.

U2 - 10.1080/00103624.2017.1282506

DO - 10.1080/00103624.2017.1282506

M3 - Article

VL - 48

SP - 576

EP - 580

JO - Communications in Soil Science and Plant Analysis

JF - Communications in Soil Science and Plant Analysis

SN - 0010-3624

IS - 5

ER -