Double-stranded library preparation for ancient and other degraded samples
Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Pennod
Fersiynau electronig
Dangosydd eitem ddigidol (DOI)
High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.
| Iaith wreiddiol | Saesneg |
|---|---|
| Teitl | Ancient DNA: methods and protocols. Second Edition |
| Cyhoeddwr | Humana New York, NY |
| Tudalennau | 65-73 |
| ISBN (Electronig) | 9781493991761 |
| ISBN (Argraffiad) | 9781493991754 |
| Dynodwyr Gwrthrych Digidol (DOIs) | |
| Statws | Cyhoeddwyd - 16 Maw 2019 |
| Cyhoeddwyd yn allanol | Ie |