Double-stranded library preparation for ancient and other degraded samples
Research output: Chapter in Book/Report/Conference proceeding › Chapter
Electronic versions
DOI
High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.
| Original language | English |
|---|---|
| Title of host publication | Ancient DNA: methods and protocols. Second Edition |
| Publisher | Humana New York, NY |
| Pages | 65-73 |
| ISBN (electronic) | 9781493991761 |
| ISBN (print) | 9781493991754 |
| DOIs | |
| Publication status | Published - 16 Mar 2019 |
| Externally published | Yes |