EDC-Mediated Oligonucleotide Immobilization on a Long Period Grating Optical Biosensor

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • X. Chen
  • C. Liu
  • M. Hughes
  • D.A. Nage
  • A.V. Hine
  • L. Zhang
e present the development and simplification of label-free fiber optic biosensors based on immobilization of oligonucleotides on dual-peak long period gratings (dLPGs). This improvement is the result of a simplification of biofunctionalization methodology. A one-step 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated reaction has been developed for the straightforward immobilization of unmodified oligonucleotides on the glass fiber surface along the grating region, leading to covalent attachment of a 5'-phosphorylated probe oligonucleotide to the amino-derivatized fiber grating surface. Immobilization is achieved via a 5'phosphate-specific linkage, leaving the remainder of the oligonucleotide accessible for binding reactions. The dLPG has been tested in different external media to demonstrate its inherent ultrahigh sensitivity to the surrounding-medium refractive index (RI) achieving 50-fold improvement in RI sensitivity over the previously-published LPG sensor in media with RI's relevant to biological assays. After functionalization, the dLPG biosensor was used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM. The proposed one-step EDC reaction approach can be further extended to develop fiber optic biosensors for disease analysis and medical diagnosis with the advances of label-free, real-time, multiplex, high sensitivity and specificity.
Iaith wreiddiolSaesneg
CyfnodolynJournal of Biosensors and Bioelectronics
Cyfrol6
Rhif y cyfnodolyn2
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 28 Meh 2015
Gweld graff cysylltiadau