Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • N Sauer
    Lehstuhl für Zellbiologische und Pflanzenphysiologie, Universität Regensburg, Regensburg
  • T Caspari
    Lehstuhl für Zellbiologische und Pflanzenphysiologie, Universität Regensburg, Regensburg
  • F Klebl
    Lehstuhl für Zellbiologische und Pflanzenphysiologie, Universität Regensburg, Regensburg
  • W Tanner
    Lehstuhl für Zellbiologische und Pflanzenphysiologie, Universität Regensburg, Regensburg

Schizosaccharomyces pombe cells were transformed with an S. pombe expression vector containing a full-length cDNA of the Chlorella hexose transporter. The transformed cells accumulated 3-O-methylglucose up to 10-fold, whereas wild-type S. pombe and control transformants could only equilibrate this sugar analogue. In a pH-jump experiment, in which extracellular pH was lowered by 1.9 units, the accumulation ratio was increased in transformed cells but not in control cells. This result indicates that the gene product, Chlorella H+/glucose-symporter protein, and a pH gradient suffice for active sugar uptake. Km values for glucose, 6-deoxyglucose, and 3-O-methylglucose of 1.5 x 10(-5) M, 2.7 x 10(-4) M, and 1.0 x 10(-3) M, respectively, were identical in Chlorella and in S. pombe cells transformed with Chlorella cDNA and approximately 100-fold lower than those of the endogenous transport system of S. pombe.

Allweddeiriau

Iaith wreiddiolSaesneg
Tudalennau (o-i)7949-52
Nifer y tudalennau4
CyfnodolynProceedings of the National Academy of Sciences of the USA
Cyfrol87
Rhif y cyfnodolyn20
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - Hyd 1990
Cyhoeddwyd yn allanolIe
Gweld graff cysylltiadau