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Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe

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StandardStandard

Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. / Sauer, N; Caspari, T; Klebl, F et al.
Yn: Proceedings of the National Academy of Sciences of the USA, Cyfrol 87, Rhif 20, 10.1990, t. 7949-52.

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

HarvardHarvard

Sauer, N, Caspari, T, Klebl, F & Tanner, W 1990, 'Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe', Proceedings of the National Academy of Sciences of the USA, cyfrol. 87, rhif 20, tt. 7949-52. https://doi.org/10.1073/pnas.87.20.7949

APA

Sauer, N., Caspari, T., Klebl, F., & Tanner, W. (1990). Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences of the USA, 87(20), 7949-52. https://doi.org/10.1073/pnas.87.20.7949

CBE

Sauer N, Caspari T, Klebl F, Tanner W. 1990. Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences of the USA. 87(20):7949-52. https://doi.org/10.1073/pnas.87.20.7949

MLA

Sauer, N et al. "Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe". Proceedings of the National Academy of Sciences of the USA. 1990, 87(20). 7949-52. https://doi.org/10.1073/pnas.87.20.7949

VancouverVancouver

Sauer N, Caspari T, Klebl F, Tanner W. Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences of the USA. 1990 Hyd;87(20):7949-52. doi: 10.1073/pnas.87.20.7949

Author

Sauer, N ; Caspari, T ; Klebl, F et al. / Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe. Yn: Proceedings of the National Academy of Sciences of the USA. 1990 ; Cyfrol 87, Rhif 20. tt. 7949-52.

RIS

TY - JOUR

T1 - Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe

AU - Sauer, N

AU - Caspari, T

AU - Klebl, F

AU - Tanner, W

PY - 1990/10

Y1 - 1990/10

N2 - Schizosaccharomyces pombe cells were transformed with an S. pombe expression vector containing a full-length cDNA of the Chlorella hexose transporter. The transformed cells accumulated 3-O-methylglucose up to 10-fold, whereas wild-type S. pombe and control transformants could only equilibrate this sugar analogue. In a pH-jump experiment, in which extracellular pH was lowered by 1.9 units, the accumulation ratio was increased in transformed cells but not in control cells. This result indicates that the gene product, Chlorella H+/glucose-symporter protein, and a pH gradient suffice for active sugar uptake. Km values for glucose, 6-deoxyglucose, and 3-O-methylglucose of 1.5 x 10(-5) M, 2.7 x 10(-4) M, and 1.0 x 10(-3) M, respectively, were identical in Chlorella and in S. pombe cells transformed with Chlorella cDNA and approximately 100-fold lower than those of the endogenous transport system of S. pombe.

AB - Schizosaccharomyces pombe cells were transformed with an S. pombe expression vector containing a full-length cDNA of the Chlorella hexose transporter. The transformed cells accumulated 3-O-methylglucose up to 10-fold, whereas wild-type S. pombe and control transformants could only equilibrate this sugar analogue. In a pH-jump experiment, in which extracellular pH was lowered by 1.9 units, the accumulation ratio was increased in transformed cells but not in control cells. This result indicates that the gene product, Chlorella H+/glucose-symporter protein, and a pH gradient suffice for active sugar uptake. Km values for glucose, 6-deoxyglucose, and 3-O-methylglucose of 1.5 x 10(-5) M, 2.7 x 10(-4) M, and 1.0 x 10(-3) M, respectively, were identical in Chlorella and in S. pombe cells transformed with Chlorella cDNA and approximately 100-fold lower than those of the endogenous transport system of S. pombe.

KW - Journal Article

U2 - 10.1073/pnas.87.20.7949

DO - 10.1073/pnas.87.20.7949

M3 - Article

C2 - 11607110

VL - 87

SP - 7949

EP - 7952

JO - Proceedings of the National Academy of Sciences of the USA

JF - Proceedings of the National Academy of Sciences of the USA

SN - 0027-8424

IS - 20

ER -