TY - JOUR

T1 - Ischaemic preconditioning in the rat brain

T2 - a longitudinal magnetic resonance imaging (MRI) study

AU - Mullins, P G

AU - Reid, D G

AU - Hockings, P D

AU - Hadingham, S J

AU - Campbell, C A

AU - Chalk, J B

AU - Doddrell, D M

N1 - Copyright -Copyright 2001 John Wiley & Sons, Ltd.

PY - 2001/5

Y1 - 2001/5

N2 - Ischaemic preconditioning in rats was studied using MRI. Ischaemic preconditioning was induced, using an intraluminal filament method, by 30 min middle cerebral artery occlusion (MCAO), and imaged 24 h later. The secondary insult of 100 min MCAO was induced 3 days following preconditioning and imaged 24 and 72 h later. Twenty-four hours following ischaemic preconditioning most rats showed small sub-cortical hyperintense regions not seen in sham-preconditioned rats. Twenty-four hours and 72 h following the secondary insult preconditioned animals showed significantly smaller lesions (24 h = 112 +/- 31 mm(3), mean +/- standard error; 72 h = 80 +/- 35 mm(3)), which were confined to the striatum, than controls (24 h = 234 +/- 32 mm(3), p = 0.026; 72 h = 275 +/- 37 mm(3), p = 0.003). In addition during lesion maturation from 24 to 72 h post-secondary MCAO, preconditioned rats displayed an average reduction in lesion size as measured by MRI whereas sham-preconditioned rats displayed increases in lesion size; this is the first report of such differential lesion volume evolution in cerebral ischaemic preconditioning.

AB - Ischaemic preconditioning in rats was studied using MRI. Ischaemic preconditioning was induced, using an intraluminal filament method, by 30 min middle cerebral artery occlusion (MCAO), and imaged 24 h later. The secondary insult of 100 min MCAO was induced 3 days following preconditioning and imaged 24 and 72 h later. Twenty-four hours following ischaemic preconditioning most rats showed small sub-cortical hyperintense regions not seen in sham-preconditioned rats. Twenty-four hours and 72 h following the secondary insult preconditioned animals showed significantly smaller lesions (24 h = 112 +/- 31 mm(3), mean +/- standard error; 72 h = 80 +/- 35 mm(3)), which were confined to the striatum, than controls (24 h = 234 +/- 32 mm(3), p = 0.026; 72 h = 275 +/- 37 mm(3), p = 0.003). In addition during lesion maturation from 24 to 72 h post-secondary MCAO, preconditioned rats displayed an average reduction in lesion size as measured by MRI whereas sham-preconditioned rats displayed increases in lesion size; this is the first report of such differential lesion volume evolution in cerebral ischaemic preconditioning.

KW - Animals

KW - Ischemic Attack, Transient

KW - Ischemic Preconditioning

KW - Longitudinal Studies

KW - Magnetic Resonance Imaging

KW - Male

KW - Middle Cerebral Artery

KW - Rats

KW - Rats, Sprague-Dawley

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/nbm.703

DO - 10.1002/nbm.703

M3 - Article

C2 - 11357186

VL - 14

SP - 204

EP - 209

JO - NMR in biomedicine

JF - NMR in biomedicine

SN - 0952-3480

IS - 3

ER -