Ischaemic preconditioning in the rat brain: a longitudinal magnetic resonance imaging (MRI) study
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In: NMR in biomedicine, Vol. 14, No. 3, 05.2001, p. 204-209.
Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Ischaemic preconditioning in the rat brain
T2 - a longitudinal magnetic resonance imaging (MRI) study
AU - Mullins, P G
AU - Reid, D G
AU - Hockings, P D
AU - Hadingham, S J
AU - Campbell, C A
AU - Chalk, J B
AU - Doddrell, D M
N1 - Copyright -Copyright 2001 John Wiley & Sons, Ltd.
PY - 2001/5
Y1 - 2001/5
N2 - Ischaemic preconditioning in rats was studied using MRI. Ischaemic preconditioning was induced, using an intraluminal filament method, by 30 min middle cerebral artery occlusion (MCAO), and imaged 24 h later. The secondary insult of 100 min MCAO was induced 3 days following preconditioning and imaged 24 and 72 h later. Twenty-four hours following ischaemic preconditioning most rats showed small sub-cortical hyperintense regions not seen in sham-preconditioned rats. Twenty-four hours and 72 h following the secondary insult preconditioned animals showed significantly smaller lesions (24 h = 112 +/- 31 mm(3), mean +/- standard error; 72 h = 80 +/- 35 mm(3)), which were confined to the striatum, than controls (24 h = 234 +/- 32 mm(3), p = 0.026; 72 h = 275 +/- 37 mm(3), p = 0.003). In addition during lesion maturation from 24 to 72 h post-secondary MCAO, preconditioned rats displayed an average reduction in lesion size as measured by MRI whereas sham-preconditioned rats displayed increases in lesion size; this is the first report of such differential lesion volume evolution in cerebral ischaemic preconditioning.
AB - Ischaemic preconditioning in rats was studied using MRI. Ischaemic preconditioning was induced, using an intraluminal filament method, by 30 min middle cerebral artery occlusion (MCAO), and imaged 24 h later. The secondary insult of 100 min MCAO was induced 3 days following preconditioning and imaged 24 and 72 h later. Twenty-four hours following ischaemic preconditioning most rats showed small sub-cortical hyperintense regions not seen in sham-preconditioned rats. Twenty-four hours and 72 h following the secondary insult preconditioned animals showed significantly smaller lesions (24 h = 112 +/- 31 mm(3), mean +/- standard error; 72 h = 80 +/- 35 mm(3)), which were confined to the striatum, than controls (24 h = 234 +/- 32 mm(3), p = 0.026; 72 h = 275 +/- 37 mm(3), p = 0.003). In addition during lesion maturation from 24 to 72 h post-secondary MCAO, preconditioned rats displayed an average reduction in lesion size as measured by MRI whereas sham-preconditioned rats displayed increases in lesion size; this is the first report of such differential lesion volume evolution in cerebral ischaemic preconditioning.
KW - Animals
KW - Ischemic Attack, Transient
KW - Ischemic Preconditioning
KW - Longitudinal Studies
KW - Magnetic Resonance Imaging
KW - Male
KW - Middle Cerebral Artery
KW - Rats
KW - Rats, Sprague-Dawley
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1002/nbm.703
DO - 10.1002/nbm.703
M3 - Article
C2 - 11357186
VL - 14
SP - 204
EP - 209
JO - NMR in biomedicine
JF - NMR in biomedicine
SN - 0952-3480
IS - 3
ER -