TY - JOUR

T1 - Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins

AU - Long, Antony R

AU - Yang, Mingyao

AU - Kaiser, Kim

AU - Shepherd, David

PY - 1998/2/27

Y1 - 1998/2/27

N2 - Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26-29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages.

AB - Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26-29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Brain/anatomy & histology

KW - Cell Cycle Proteins/biosynthesis

KW - Cloning, Molecular

KW - Drosophila/genetics

KW - Drosophila Proteins

KW - Genes, Insect

KW - Genes, Reporter

KW - Heterozygote

KW - Homozygote

KW - Larva

KW - Molecular Sequence Data

KW - Neurons, Afferent/metabolism

KW - Recombinant Fusion Proteins/biosynthesis

KW - Sequence Alignment

KW - Sequence Homology, Amino Acid

U2 - 10.1016/S0378-1119(97)00652-5

DO - 10.1016/S0378-1119(97)00652-5

M3 - Article

C2 - 9524263

VL - 208

SP - 191

EP - 199

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -